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QIAGEN Launches Standardized Solutions for Molecular Analysis of FFPE Samples

QIAGEN
5 Nov 2010
Sarah Sarah
Marketing / Sales

Product news

QIAGEN has launched several new products that assist in the molecular analysis of formalin-fixed, paraffin-embedded (FFPE) tissue samples. The new offering provides scientists with a range of dedicated kits over the whole FFPE workflow, from the isolation of DNA and total RNA, the setup of RT-PCR reactions through to bisulfite kits for applications in epigenetics research.

The portfolio extension is supported by a new brochure “Unlocking your FFPE Archive” that describes critical factors for molecular analysis of FFPE samples from preparation and storage to retrieval and analysis, as well as downstream applications such as reverse transcription and real-time PCR.

The new kits include the AllPrep DNA/RNA FFPE Kit for purification of DNA and RNA from the same sample, the RNeasy® FFPE Kit for gDNA free isolation of RNA, as well as the miRNeasy FFPE Kit for the isolation of miRNA. The new QuantiFast Probe RT-PCR kits are tailored for gene expression analysis of FFPE samples with highly fragmented starting material and the EpiTect Plus Bisulfite Kits for epigenetics research. A deparaffinization solution also has been launched that is used to prepare the samples prior to purification of nucleic acids.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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