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Proteomics on NGS: Game-changing technology to increase multiplexing and throughput

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Join us on Monday, June 22, to learn how Olink’s latest immunoassay technology is taking proteomics to a new level

11 Jun 2020
Tom Casburn
Associate Editor

Editorial article

Dr. Ida Grundberg and Dr. Erika Assarsson, Olink Proteomics

Protein biomarkers are increasingly used in clinical research to identify protein signatures that can predict outcomes, stratify patient populations, and provide insights into disease biology. Proteomics technologies have been restricted, however, by limitations in throughput, multiplexing level, specificity, and sensitivity.

Recent developments in multiplex technologies that enable high-throughput analysis of many proteins simultaneously, while using minimal volumes of biological samples, are accelerating the utility of proteomics.

Olink Proximity Extension Assay (PEA) technology featuring dual-recognition with matched DNA oligo-linked antibody pairs and qPCR readout has already been used to generate >160 million protein data points and ~400 peer-reviewed publications. Newly launched, Olink® Explore 1536 now takes this to an entirely new level, with PEA coupled to next-generation sequencing (NGS) readout enabling 1,472 proteins to be measured with just 3 µL plasma/serum and a throughput of 1.3 million protein data points/week on one instrument.

In this upcoming SelectScience webinar, Dr. Ida Grundberg, Chief Scientific Officer, and Dr. Erika Assarsson, Head of R&D, Olink Proteomics, explore how this technology could be a game changer for proteomics.

Register Now


Key learning objectives:

  • What is Olink Explore 1536
  • What are the benefits of PEA on NGS

Who should attend:

  • Scientists with an interest in proteomic research who have access to samples
  • Pharma/biotech, clinical and academic researchers
  • Clinical research mass spec users running blood samples
  • Research groups working with biobanks
  • Researchers working with large scale epidemiology

The live webinar takes place on Monday, June 22, 2020, at:

  • 16:00 BST
  • 17:00 CEST
  • 11:00 EDT
  • 08:00 PDT

Register to watch the full webinar here>>

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars>>

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.NGSProtein BiologyBiomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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