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Protein Forest Inc. Launches Digital ProteomeChip™ System for Mass Spectrometry Sample Preparation and Data Analysis

Protein Forest, Inc.
1 Jun 2008

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Protein Forest, Inc. a leading developer of state-of-the-art sample fractionation technology for Mass Spectrometry and data analysis software, announced today that it will launch the digital ProteomeChip™ System at the ASMS meeting in Denver, CO on June 1. The ProteomeChip™ technology fractionates proteins or peptides by parallel isoelectric focusing on dPC™ chips for mass spec analysis and western blotting and the Mass Spec Results and Analysis Tool™ (MSRAT™) bioinformatics software allows the researcher to discover the richness of their dPC™ data produced.

"We are very excited about launching our digital ProteomeChip System as it addresses critical limitations of existing fractionation and sample prep technologies available to researchers today, particularly those that utilize mass spectrometry," said Russell Garlick, Protein Forest’s President and Chief Executive Officer. "dPC™ chips are precision manufactured to have a difference of 0.05 pH units between adjacent gel plugs with guaranteed reproducibility which allows proteomic scientists the ability to fractionate and concentrate their samples using parallel isoelectric focusing. This technology enables the separation and identification of proteins or peptides often not found by other techniques.”

About the digital ProteomeChip System
The dPC™ technology easily integrates into existing mass spectrometry workflows. Simply reduce, alkylate and desalt samples before adding to the dPC™ running buffer. Separate for 30 minutes on the dPC™ Chip and protein samples are ready for in-gel tryptic digestion followed by mass spec identification. The dPC™ technology is compatible with a wide variety of mass spectrometry instrumentation. Alternatively, proteins can be identified by immunoblotting. The combination of the simple workflow and precise dPC™ manufacturing process insures reproducibility from chip to chip and experiment to experiment.

MSRAT™ bioinformatics software is used to rapidly analyze large amounts of mass spec data within minutes. This software helps the researcher quickly focus on differences between samples including pI isoforms, post-translational modifications and differential expression. This software helps researchers to quickly identify important biologically relevant features from their proteomic experiments and it also streamlines QC and validation for bioprocess manufacturing.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Lab-on-a-ChipLab-on-a-chip (LOC) technology uses microfluidic chips, such as microarrays, to perform biochemical assays on a small scale. Benefits of lab-on-a-chip include the small volume of sample required as well as the speed of analysis. In selecting lab-on-a-chip devices consider the assay, cost, reliability, sensitivity and signal to noise ratio.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.Chem / BioinformaticsCheminformatics and bioinformatics are computational techniques used in chemistry and biology, respectively, for data acquisition, processing and storage. Cheminformatics focuses on compound information, whereas bioinformatics is mainly applied to analysis and modeling of genomics, genetic and sequencing information. Hardware and software is available for data acquisition, analysis, management and storage.Molecular Recognition SoftwareMolecular recognition software is widely used to analyze DNA, RNA, proteins and chemicals. The software can be useful for graphical viewing, comparative analyses, high-throughput screening, genomics, proteomics and phylogenetics. Molecular recognition software uses bioinformatics tools and analyses such as BLAST searches and generates structural predictions, 3D structures and sequencing information.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.

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Protein Forest Inc. Launches Digital ProteomeChip™ System for Mass Spectrometry Sample Preparation and Data Analysis