Promega ViaFect™ Transfection Reagent Delivers High Performance Transfection across Multiple Cell Lines, Including iPS Cells

17 Jan 2014
Sarah Thomas
Associate Editor

Product news

Promega Corporation announces ViaFect™ Transfection Reagent, a tool for efficient DNA transfer into a wide variety of cell lines, including challenging suspension cells and stem cell-derived lines. ViaFect Transfection Reagent allows researchers to develop transfection-based assays in the cell line most relevant for their biology of interest.

Induced pluripotent stem cell (iPSC)-derived cell models, such as Cellular Dynamics International's iCell® Cardiomyocytes, show robust transfection with ViaFect Transfection Reagent, enabling assays not possible with other reagents.

This low toxicity reagent allows cells to stay healthy and metabolically active during transfection-based experiments, even at low cell densities, facilitating experiments where extended application of a treatment is needed. Cell viabilities of greater than 90% are routinely observed following transfection with ViaFect Transfection Reagent.

ViaFect Transfection Reagent requires minimal optimization with a simple three-step protocol. It does not require removal of serum, removal of culture medium or washing after introduction of the reagent/DNA complex. ViaFect Transfection Reagent contains no animal-derived components.

For more information or to sample the new reagent, click on the company website link below. A poster detailing results using ViaFect Transfection Reagent will also be presented at the Society for Laboratory Automation and Screening Conference and Exhibition to be held January 18th-22nd in San Diego, CA.

ViaFect™ Transfection Reagent

Promega Corp.

ViaFect™ Transfection Reagent allows high-efficiency transfection of a wide range of cell types, including iPS Cells, without compromising cell viability.   ViaFect™ Transfection Reagent enables the design of biologically relevant assays in workhorse adherent cell models, suspension cells important for studying cell signaling, and even differentiated cell lines derived from stem cells. ViaFect™ Transfection Reagent offers an easy-to-use protocol that provides superior performance with minimal optimization, allowing you to design the right assay in the right cells to model the biology you are studying. ViaFect Transfection Reagent requires minimal optimization with a simple three-step protocol. It does not require removal of serum, removal of culture medium or washing after introduction of the reagent/DNA complex. ViaFect Transfection Reagent contains no animal-derived components.  

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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.SLASThe Society for Laboratory Automation and Screening (SLAS) is an organization focused on laboratory automation, high-throughput screening, and biotechnology innovation. SLAS promotes scientific advancements through conferences, publications, and industry collaborations. IpscTransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.