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Promega ProteasMAX improves protein characterization

Promega Corp.
21 May 2008

Product news

Promega Corporation, a leading provider of trypsin protease to the life sciences industry, announces the launch of ProteasMAX™ Surfactant. ProteasMAX has been designed to enhance the enzymatic performance of trypsin and other proteases in preparation for analysis by mass spectrometry or liquid chromatography. The surfactant enables scientists to obtain a more complete digestion prior to protein analysis, resulting in more accurate data in a shorter time, and reduced risk of sample degradation.

ProteasMAX Surfactant delivers both on process and results with the following benefits:

  • Confidence in data – improves protein digestion, exposing cleavage sites usually inaccessible due to secondary or tertiary structure
  • Improved sensitivity – increases sample recovery from gels
  • Higher productivity – standard protein digestion time can be reduced from overnight to just three hours
  • Sample quality preservation – enhances protein solubilization at room temperature, which means that high temperatures (and precipitation) can be avoided, even for complex proteins

Because ProteasMAX degrades during the digestion reaction, researchers can proceed directly to analysis of peptides by mass spectrometry without additional inactivation steps. The new reagent is also compatible with existing protocols for 1 or 2D in-gel digestion of a purified protein or the digestion of complex protein samples by 2D LC-MS/MS.

“We perceive a growing demand for higher throughput and streamlined protocols in proteomics”, said Dr.Hrissi Samartzidou, Global Director for Gene and Protein Sciences. “Our strategy is to identify process bottlenecks and the needs of proteomics researchers in order to develop the optimum solutions to address them.”

As a supplier to protein research for over a decade, Promega plans to continue to make innovative contributions such as ProteasMAX that will fulfill the complex needs of scientists’ in the area of Proteomics sample prep with focus on protein sample depletion, enrichment and digestion.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.

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Promega ProteasMAX improves protein characterization