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Promega GoTaq Probe Real-Time PCR Systems Provide Sensitive Quantitation with Resistant-to-Inhibitor Master Mixes

Promega Corp.
17 Oct 2012

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Promega Corporation has announced the launch of the GoTaq® Probe PCR Master Mix, GoTaq Probe 1-Step RT-qPCR System, and GoTaq Probe 2-Step RT-qPCR System. These ready-to-use, 2x master mixes simplify the preparation of reactions for qPCR using hydrolysis probe detection, and are designed for sensitive sequence detection and quantification over a broad range of DNA or RNA targets, gene expression analysis, detection of sequence variants, and fluorescent detection.

Rapid hot-start activation and enzymes make GoTaq Probe Systems compatible with any real-time PCR instrument using standard or fast cycling programs. The 1-step and 2-step systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. These Systems are compatible with other probe assays such as molecular beacons.

GoTaq Probe qPCR Master Mix, designed for qPCR from gDNA templates, provides resistance to a wide range of PCR inhibitors. Using antibody-mediated hot-start chemistry, reaction set-up can be performed at room temperature. The master mix does not contain a reference dye, however carboxy-X-rhodamine is included with the system, to be added to amplification reactions if desired.

GoTaq Probe 1-Step RT-qPCR System combines GoScript Reverse Transcriptase and GoTaq Probe qPCR Master Mix in single-step, real-time amplification reactions for the detection and relative quantification of RNA expression levels using a one-step RT-qPCR method.

GoTaq Probe 2-Step RT-qPCR System enables detection and relative quantification of RNA expression levels via a two-step RT-qPCR method, using the integrated components of the GoScript Reverse Transcription System and GoTaq Probe qPCR Master Mix. GoScript Reverse Transcription System includes an optimized reaction buffer and reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product may be added directly to downstream qPCR amplification reactions.

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GoTaq® Probe 2-Step RT-qPCR System

Promega Corp.

The GoTaq® Probe 2-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system protocol facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: • GoScript™ Reverse Transcription System • GoTaq® Probe qPCR Master Mix The GoScript™ Reverse Transcription System includes an optimized reaction buffer and reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product may be added directly to downstream qPCR amplification reactions. The GoTaq® Probe qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add reference dye to amplification reactions if desired. Features - Benefits • Superior Performance: Sensitive detection on any real-time instrument • Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection • Versatility: Compatible with both fast and standard cycling methods • Robustness: High-efficiency, full-length cDNA synthesis in the presence of inhibitors • Confidence: The Promega PCR Performance Guarantee Applications • Sensitive sequence detection and quantification • Gene expression analysis • Detection of sequence variants • Fluorescent detection

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GoTaq® Probe 1-Step RT-qPCR System

Promega Corp.

  The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add reference dye to amplification reactions if desired. The GoTaq® Probe qPCR Master Mix is designed to provide resistance to a wide range of PCR inhibitors. This formulation uses antibody-mediated hot-start chemistry, allowing reaction setup to be performed at room temperature. The master mix also employs rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs. Features - Benefits • Superior Performance: Sensitive detection on any real-time instrument • Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection • Versatility: Compatible with both fast and standard cycling methods • Robustness: High-efficiency, full-length cDNA synthesis in the presence of inhibitors • Confidence: The Promega PCR Performance Guarantee Applications • Sensitive sequence detection and quantification • Gene expression analysis • Detection of sequence variants • Fluorescent detection  

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GoTaq® Probe qPCR Master Mix

Promega Corp.

The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format.   It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe). This master mix does not contain a reference dye; however, a separate tube of carboxy-X-rhodamine (CXR) reference dye is included with this system, allowing users to add reference dye to amplification reactions if desired. The GoTaq® Probe qPCR Master Mix is designed to provide resistance to a wide range of PCR inhibitors. This formulation uses antibody-mediated hot-start chemistry, allowing reaction setup to be performed at room temperature. The master mix also employs rapid hot-start activation and processive enzymes, making it compatible with both standard and fast instrument cycling programs. Features - Benefits • Superior Performance: Sensitive detection on any real-time instrument • Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection • Versatility: Compatible with both fast and standard cycling methods • Robustness: High-efficiency, full-length cDNA synthesis in the presence of inhibitors • Confidence: The Promega PCR Performance Guarantee Applications • Sensitive sequence detection and quantification • Gene expression analysis • Detection of sequence variants • Fluorescent detection  

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