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µ-PrepCell™ Synthesizer for Metabolites

Antec Leyden BV
22 Sept 2011
Roger Wayman
Administrator / Office Personnel

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Antec have introduced a new synthesizer for metabolites called µ-PrepCell™. It’s designed to be a quick and clean alternative for in-vivo studies. It can synthesize micrograms of metabolites and collect fractions for MS or NMR.

Structural elucidation of reactive metabolites plays an important role in drug development. Xenobiotics such as drugs are often metabolized by cytochrome P450 enzymes (CYP) to oxidative metabolites. It has been shown that the vivo formation of these metabolites can be mimicked in vitro by electrochemical oxidation in a flow cell. Generating these metabolites in vitro has a number of advantages over in vivo formation, most important: it is much quicker and cleaner. Metabolites are formed instantaneously in a well defined matrix.

The µ-PrepCell is designed to synthesize micrograms of metabolites in a short period of time. The active surface of the working electrode is about 10 times the size of a standard cell, and guarantees a significantly higher efficiency in formation of reactive metabolites. The cell can be used in the ROXY EC system for off-line collection of metabolites. Flow rate and working potential can be optimized using the Dialogue software. The µ-PrepCell has a diamond film electrode which facilitates high working potentials up to 4.9 Volt for even the most demanding reaction conditions. The HyREF reference electrode is maintenance free and the stainless steel spacer is convenient to handle.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. NMR and EPR SpectroscopyNuclear magnetic resonance (NMR) spectroscopy is used to resolve the local chemical environment of atomic nuclei with spin, revealing information on molecular structure, dynamic processes and chemical reactions of organic molecules, from proteins to synthetics. Electron paramagnetic resonance (EPR) also known as electron spin resonance (ESR) spectroscopy is used to detect and quantify paramagnetic species in a sample, including free radicals as transition metal ions. By immersing the sample in a strong magnetic field, both NMR and EPR spectrometers probe the sample with either radio waves or microwaves respectively. A range of benchtop, solid-state and time domain NMR spectrometers & EPR spectrometers are available, as well as NMR tubes, NMR solvents, software, coils, and magnets. Find the best NMR & EPR equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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µ-PrepCell™ Synthesizer for Metabolites