Precise Imaging of Stain-Free Protein Gels

13 Jan 2017

Emily Adam

Publishing / Media

Product news

Syngene, a world-leading manufacturer of image analysis solutions, is delighted to announce its GeneSys software has been upgraded to include an icon of pre-set optimized “stain-free” protein gel imaging conditions. This simple to use software, available to download free of charge to existing users of specified Syngene imaging systems, will save set-up time and ensure accurate stain-free protein gel images every time.

For use in Syngene’s G:BOX Chemi and PXi imaging systems, the new GeneSys software now includes a “stain-free” imaging icon. The icon has been set up using optimum filter and lighting conditions that Syngene’s technical specialists have determined can accurately detect nanogram levels of protein on a Stain-Free gel. Using this new software, TGX Stain-Free™ and Criterion™ TGX Stain-Free™ precast gels can be rapidly imaged, saving researchers the time and effort of looking up recommended detection conditions, as well as gel staining preparation time.

The new software version maintains its excellent user-friendly features including guiding scientists through protocol set-up using its database of imaging conditions for hundreds of commercially available dyes and stain-free options. This allows users of Syngene’s high specification imaging systems to visualize dyes such as Coomassie Blue, GelGreen™, SYBR® Gold, SYBR® Safe and SYPRO® Ruby. The software calibrates to each gel or blot’s size to generate high-quality images. For faster image capture, GeneSys also has a unique researcher ‘protocol save’ capability enabling one-click recall of frequently used settings.

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The biggest difference between a Syngene system and any other is in the way the system is controlled. At the heart of all Syngene systems is GeneSys which is an application driven control software. You can use GeneSys in either Automatic or Manual mode. It is assumed that the user will know exactly what their application is and how they have prepared their gel or blot. In Auto Mode they simply enter this information into GeneSys (or recall it from a saved configuration) and the system takes over the rest. Behind GeneSys is an extensive database which contains data relating to a very wide range of applications, eg, fluorescence, chemiluminescene and chemifluorescence.Once the imaging system has been told what sample to expect then GeneSys decides what hardware configuration is best and sets the system ready for image capture. Things like camera control, exposure time, sensitivity setting, lighting requirements, lens control, filter selection – all of this is taken care of by GeneSys. The user just has to click the ‘capture’ button and wait for the perfect image to appear. GeneSys ensures scientists can quickly capture excellent images of even complex multiplex gels.The innovative GeneSys software features large touch-screen buttons which guide users effortlessly through set-up and image capture. Each screen prompts researchers to select, for example, the type of gel or blot they are using and what it is stained with.
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For high performance imaging, the new generation of G:BOX Chemi gel doc systems provide the user with an extended range of applications covering fluorescence and chemiluminescence. All these systems now utilise Syngene’s groundbreaking GeneSys automatic control software. Driven from a database containing hundreds of capture protocols, full manual control is available should you wish to use it. The G:BOX Chemi series features cameras with resolutions of 4m, 6m or 9m pixels. All have cooling which reduces the small amount of electronically generated noise to produce outstanding images with minimal background interference. These new generation cameras also have higher quantum efficiencies (QE) than most, helping the system to achieve outstanding levels of sensitivity. Whether using the system for standard fluorescence applications such as DNA, or for chemiluminescence imaging, the G:BOX Chemi will give you high quality results. In addition, by using the optional LED modules users can work with a unique automated multiplexed function capable of using up to 5 channels. Other LED modules can be used to turn the G:BOX Chemi into a highly sensitive IR capture system. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels. The stylish design with modular construction includes motor-driven lenses, filter wheels with the option of including a range of lighting choices for both Epi and transillumination applications. Syngene has just introduced the new high intensity HI-LEDs option for the G:BOX range, resulting in superior imaging performance for your fluorescence applications. Check the status of an image capture and access your recently captured images from any computer, smartphone or tablet on the same network as G:BOX Chemi XT4 gel doc system using the new "Status Link" feature. This is ideal for any busy laboratory. The system comes complete with unlimited copies of GeneTools analysis software.
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Gel Doc / Image AnalysisProtein PurificationSoftware PlatformsGel ImagingProtein BiologyProtein Detection

G:BOX Chemi XT4

Syngene

For high performance imaging, the new generation of G:BOX Chemi gel doc systems provide the user with an extended range of applications covering fluorescence and chemiluminescence. All these systems now utilise Syngene’s groundbreaking GeneSys automatic control software. Driven from a database containing hundreds of capture protocols, full manual control is available should you wish to use it. The G:BOX Chemi series features cameras with resolutions of 4m, 6m or 9m pixels. All have cooling which reduces the small amount of electronically generated noise to produce outstanding images with minimal background interference. These new generation cameras also have higher quantum efficiencies (QE) than most, helping the system to achieve outstanding levels of sensitivity. Whether using the system for standard fluorescence applications such as DNA, or for chemiluminescence imaging, the G:BOX Chemi will give you high quality results. In addition, by using the optional LED modules users can work with a unique automated multiplexed function capable of using up to 5 channels. Other LED modules can be used to turn the G:BOX Chemi into a highly sensitive IR capture system. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels. The stylish design with modular construction includes motor-driven lenses, filter wheels with the option of including a range of lighting choices for both Epi and transillumination applications. Syngene has just introduced the new high intensity HI-LEDs option for the G:BOX range, resulting in superior imaging performance for your fluorescence applications. Check the status of an image capture and access your recently captured images from any computer, smartphone or tablet on the same network as G:BOX Chemi XT4 gel doc system using the new "Status Link" feature. This is ideal for any busy laboratory. The system comes complete with unlimited copies of GeneTools analysis software.

(1)

Precise Imaging of Stain-Free Protein Gels

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