NewsSeparations
PhyTip Columns: Beyond Affinity Purification - Adapting Ion Exchange to Small-Scale Columns
9 Sept 2013Product news
The newest separation chemistry to be adapted into the PhyTip column is ion exchange. In traditional ion exchange chromatography, samples, wash buffers and gradient elution buffers pass through packed columns in one direction. The PhyTip column is able to simulate this chromatographic separation in a small, pipette tip column by driving the binding and release steps to equilibrium. This is not achieved by using high back-pressure, large columns, but instead by PhyNexus’ patent pending back-and-forth flow.
Related products
Request Quote for All Products
PhyTip® Columns and MiniPrep PhyTip® Columns
BiotagePhyTip® columns use dual-flow chromatography within pipette tips to automate purification workflows. This technology delivers high reproducibility and exceptional sample purity for efficient, reliable results.
Tags
Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Antibody Purification