SelectScience
Join Free
Accelerating Science
NewsSeparations

PhotoChemical HPLC PCR of AflaToxins

ARC Sciences
13 Apr 2009
Samantha Rosoman
Campaign Coordinator

Product news

The UVETM-derivatiser is a professionally designed device for the photochemical post-column derivatisation of aflatoxins. The derivatisation is performed in a special reactor loop with UV-light. No chemicals are required.

Just as in the conventional post-column derivatisation using iodine or bromine, aflatoxins B1 and G1 are transformed to stable fluorescent derivatives. Here, the water present in the HPLC-eluant is used as a reagent. Consequently, neither iodine nor HNO3/KBr are used. The results are clear peaks and a high derivatisation performance. The application is simple and easy to perform: Simply fit UVETM between HPLC and detector, switch on the equipment – done! For more information contact ARC Sciences.

• Simple Plug and Play Device
• Low Cost, less than ½ the price of chemical PCRs
• No Chemicals required, only uses light to derivatise the analytes
• Works with all HPLC systems
• Results certified as equivalent to Post Column Derivatisation using Iodine or Bromine

Links

ARC SciencesCompany website

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.

Related articles

PhotoChemical HPLC PCR of AflaToxins