Phire™ Hot Start DNA Polymerase from Finnzymes – Speed and specificity for routine and high-throughput PCR

11 Mar 2008

Product news

Finnzymes has launched a novel hot start DNA polymerase that outperforms every Taq-based hot start polymerase on the market.

Phire Hot Start DNA Polymerase incorporates a DNA-binding domain, a design that results in shorter extension times, improved yields, and 2-fold increased fidelity compared to Taq polymerase. In addition, our unique hot start technology allows complete reactivation of Phire DNA Polymerase in “zero-time” at standard cycling temperatures. Finnzymes’ new hot start polymerase is an ideal solution for routine and high-throughput PCR applications.

Advantages:
Short protocols – Amplify 3 times faster than with hot start Taq
Specific products – Unique hot start technology with zero-time reactivation
High yields – Efficient PCR results in more product

Phire™ Hot Start II DNA Polymerase

Finnzymes Oy

Finnzymes’ Phire™ Hot Start DNA Polymerase is a novel PCR enzyme. The polymerase incorporates a specific DNA-binding domain which shortens extension times, improves yields, and increases fidelity 2-fold compared to Taq polymerase. In addition, it our unique hot start technology allows complete reactivation of Phire DNA Polymerase in “zero-time” at standard cycling temperatures. This combination of features makes this polymerase an ideal solution for routine and high throughput PCR applications. Phire DNA Polymerase delivers superior performance in all thermal cyclers, both conventional and fast instruments such as the Piko™ Thermal Cycler from Finnzymes. Advantages• Quick hot start: No reactivation step• Fast enzyme: Amplify 3 times faster than with hot start Taq• High yields: Abundant products due to high efficiency• Longer PCR products: Amplify significantly longer DNA fragments than with any hot start Taq

(0)

Links

Tags

Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.