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PerkinElmer Offers Enhanced Software for Multiplex Quantitative Protein Expression Profiling

PerkinElmer
4 Jun 2007

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At the American Society for Mass Spectrometry (ASMS) Annual Conference, PerkinElmer Life and Analytical Sciences, a global leader in life science research, drug discovery and cellular science, announced that it has introduced a software upgrade for its patented ExacTag™ technology, an innovative, multiplex platform for quantitative protein expression profiling by mass spectrometry (MS).

ExacTag Analysis Software version 2.0 offers powerful new data visualization and analysis tools, and supports many file formats for wide MS system compatibility. The new 2.0 software is included with ExacTag™ Labeling Kits enabling simultaneous comparison of 2, 4, 7, or 10 samples using any tandem mass spectrometer.

“Multiplex protein labeling and detection coupled with a powerful, versatile Mass Spec analysis software package represent an essential toolset for protein research labs,” said Dr. Richard Eglen, vice president and general manager, Discovery and Research Reagents for PerkinElmer Life and Analytical Sciences. “When measuring quantitative changes in protein expression, the simultaneous sample analysis afforded by multiplexing contributes to greater consistency and reproducibility of the measurements, as well as greater lab productivity, while the advanced software package gives the researchers highly-relevant, functional insights into their data.”

ExacTag Analysis Software uses signal intensity measurements from low mass reporter ions generated in the collision cell of a tandem mass spectrometer and protein identification data from standard databases like Mascot™ or SEQUEST® to quantify and identify proteins of interest. Version 2.0 software offers researchers the full spectrum of data resolution and more data visualization and manipulation options to hone in or cluster results, and render as visual heat maps.

ExacTag Labeling Kits utilize a family of thiol and amine reactive isobaric mass tags that offer flexibility for the quantification of protein expression of up to 10 samples simultaneously. Researchers can label samples at the protein level and then pool for fractionation/separation to allow for greater complexity reduction and thus higher selectivity, sensitivity and precision in quantitative protein analysis.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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PerkinElmer Offers Enhanced Software for Multiplex Quantitative Protein Expression Profiling