PerkinElmer Introduces New Improved Luminescence Assay Kits

17 Apr 2007

Product news

PerkinElmer, Inc., a global leader in Health Sciences and Photonics, has developed two new luminescent gene reporter assays kits, steadylite plus™ and britelite™ plus, which deliver industry-leading speed and sensitivity in new conveniently-stored, odor-free formulations.

“The new steadylite plus and britelite plus offer high-throughput screening laboratories the same performance and reliability of our current luminescent reporter gene reagents, and are further proof of our continued commitment to developing our reagent tool box for GPCR research,” says Dr. Richard Eglen, vice president and general manager, Discovery & Research Reagents, for PerkinElmer Life and Analytical Sciences. “These new kits can be conveniently stored at refrigerated temperatures instead of freezer temperatures so they can be used right away without thawing and our odor-free reagents eliminate the need for working in hoods.”

steadylite plus and britelite plus are highly sensitive firefly luciferase reporter gene assay kits for the quantification of gene expression in cells. Cell-based assays, especially for high value GPCR targets, are increasingly critical to pharmaceutical drug discovery screening.

With its long-lasting signal, steadylite plus is designed for batch-processing systems. britelite plus is designed for continuous process systems. Both steadylite plus and britelite plus kits are suitable for high-density microplates such as 384- and 1536-well plates in high throughput environments. steadylite plus can also be used for smaller-scale sample analysis in 96-well plates when higher sensitivity is required.

PerkinElmer’s portfolio of luciferase-based assays with a broad dynamic range feature optimized reagents for highly specific HTS screens. In addition to reporter gene assays, PerkinElmer offers ATPlite™ cytotoxicity/cell proliferation assays and easylite™ kinase assays. steadylite plus and britelite plus assay kits are compatible with the leading luminescent detection instruments including PerkinElmer’s high-performance ViewLux™, EnVision™, Fusion™, and TopCount® readers.

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High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.