Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader

Promega has developed reporter bioassays that use activation of gene transcription through NFAT (nuclear factor of activated T-cells) as an earlier measure of ADCC. The reporter bioassays provide a convenient system for assessing ADCC that uses frozen, thaw-and-use effector and target cells and eliminates the need for cell culture maintenance. This application note demonstrates the Infinite® 200 PRO Reader with Gas Control Module to be functionally equivalent to a tissue culture incubator for the reporter induction phase of the ADCC Reporter Bioassay.