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Perfinity iDP – Online Protein Digestion Within a Few Minutes

9 Apr 2014
Sarah Thomas
Associate Editor

Product news

Shimadzu, one of the world leaders in analytical instrumentation, offers the Perfinity iDP system for automated online protein digestion. The heart of this system solution, which is based on ultra-high performance liquid chromatography (UHPLC), is a reusable high-performance trypsin column that, depending on the protein, can per-form a digestion within a few minutes.

Automated online tryptic digestion
The Perfinity iDP is a complete system for tryptic digestion of proteins that minimizes potential user-related errors. The sample is applied onto the high-performance trypsin column using an autosampler and is digested in a buffer that is optimized for this application. The peptides resulting from the digestion are transferred online to a desalting column and are subsequently directed to the separation column where they are separated and subsequently analyzed. This completely automated process ensures an excellent recovery with virtually no sample carry-over. Parallel processing using two desalting columns further increases sample throughput.

Simple operation via the Perfinity iDP software
The Perfinity iDP software supports the entire workflow, from method development up to sample analysis. Results are obtained in only three intuitive steps – method setting, batch file creation, evaluation via LabSolutions. By connecting with Shimadzu’s LabSolutions software, the Perfinity iDP can be easily integrated into already existing systems.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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Perfinity iDP – Online Protein Digestion Within a Few Minutes