pDG helper & packaging vectors for AAV production

Vectors such as pDG, pDM, pDP1rs to pDP6rs and pDG without a heparin binding motive are immediately available from PlasmidFactory.

The vectors are produced in reproducible quality by PlasmidFactory´s standardized manufacturing technology, which includes LPS-endotoxin removal and cultivation without any animal derived component. Homogeneous products of precisely defined quality are assured by the proprietary CGE analysis technology. The service includes the complete characterization of the products by LAL test (LPS-endotoxin), BCA test (total protein), UV spectroscopy, restriction analysis, detection of RNA and bacterial chromosomal DNA using AGE, and sequencing (partial).

Co-transfection of cells with pDG and AAV vector plasmids (two plasmid system) is sufficient for the reproducible production of infectious recombinant AAV, since the pDG vectors contain all AAV and adenovirus functions required for amplification and packaging of AAV vector plasmids. Triple-transfection is no longer necessary. Furthermore, super-infection with a helper virus – in contrast to traditional systems – is also dispensable. For optimal co-transfection results homogenous, intact supercoiled plasmid DNA is a prerequisite, which is manufactured up to GMP Grade Quality by PlasmidFactory.

The pDG vectors are manufactured under license from the German Cancer Research Centre (DKFZ), Heidelberg.