Papain Kit For Effective Neural Cell Isolation

30 Sept 2012

Product news

Visit Worthington booth #2813 at the Society for Neuroscience 2012 Annual Meeting in New Orleans, LA. to complete a short ʻPrimary Neural Cell Isolationʼ survey and enter a draw to win an Apple iPod.

Worthington's 'Papain Dissociation System' provides researchers with an all inclusive kit to isolate neural cells with consistently high yield and viability. This kit utilizes Worthington’s purified papain and contains all the necessary materials and directions for five separate tissue dissociations. Individual vials of each contain Earle's balanced Salt Solution (EBSS), Worthington Purified Papain, DNase and Ovomucoid Inhibitor-BSA, an inhibitor and single density gradient for cell puri fication. Also included are a phenol red color card for simple monitoring of pH and a detailed protocol. To ensure consistent results the kit uses purified enzymes rather than crude preparations and each lot is functionally use-tested for rat pup spinal cord neurons.

Also request a copy of Worthington's NEW Tissue Dissociation Guide that is available to assist researchers with selecting enzymes, developing and optimizing protocols and better understanding the process of enzymatic primary neural & other types of cell isolation. The guide discusses the enzymes most commonly used, their handling and specificity, and includes hundreds of new detailed cell and species-specific references.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NeuroscienceNeuroscience research investigates the neurological mechanisms underlying behavior, neurodegenerative diseases, and other brain conditions. Learn about the innovative technologies for bioimaging, electrophysiology, cell culture, chromatography and other techniques used in this field.Tissue Dissociation
Papain Kit For Effective Neural Cell Isolation