Pall Life Sciences Exhibits New Solutions for HPLC Sample Prep at Pittcon 2013

Pall Life Sciences - Laboratory, Food, Beverage

4 Mar 2013

Product news

Analytical sample prep is growing, facilitating a movement to analyses of smaller samples with more sensitive assays and a need to process more samples faster. To that end, Pall Life Sciences has introduced multiple new products that are used in HPLC sample prep.

End users expect analytical sample prep to have low extractable contributions, low analyte adsorption, and the ability to filter very small samples. Pall Life Sciences will be featuring their recently launched 13 mm Acrodisc® MS syringe filter certified for low extractables in high performance liquid chromatography/mass spectrometry (LCMS) applications. Designed specifically for small sample analysis using LCMS, the 13 mm Acrodisc MS syringe filter improves the accuracy of testing, enhances LCMS performance, and extends the longevity of testing instrumentation. We will be featuring a poster (2190-3P) on Wednesday afternoon of the show highlighting the benefits of this exciting new product line. More information about this new product can be obtained at the Pall Life Sciences booth 1349.

Sample prep automation is also moving forward with high throughput processing using both 96-well and 384-well plate filtration products. In addition, there are also systems available for simultaneously filtering a complete set of samples directly into a carousel for an HPLC system. These types of systems allow automation of the filtration process so it can be completed in-batch or in-process via automation. In addition to our 13 mm Acrodisc MS syringe filter, Pall offers AcroPrep™ Advance filter plates, and Microsep™ Advance and Macrosep® Advance centrifugal devices to round out our offering.

Pall is a filtration, separation and purification company that provides solutions to meet the critical fluid management needs of the life sciences industry. The company's engineered products enable process and product innovation and minimize emissions and waste. Pall’s products are used routinely in HPLC sample preparation.

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AcroPrep™ Advance Filter Plates
Pall Life Sciences - Laboratory, Food, Beverage
AcroPrep™ Advance filter plates are the newest edition to Pall’s filter plate family. New sealing technology and advanced tip design make these the first choice in critical sample prep applications. Aplications: Lysate Clearance DNA purification Protein purification Multiplex assays Aqueous filtration Particulate removal Size exclusion Bead-based assays Neonatal screening Flow cytometry Solvent filtration Concentration, purification and desalting of proteins and nucleic acids Features: Smooth well design – Provides consistency in filtration times, as well as efficient sample and bead recovery Optimized outlet tips – Minimizes sample leakage during incubation steps and reduces the presence of hanging drops following filtration New well geometry – Results in faster, more uniform filtration rates across the plate, thereby improving well-to-well, plate-to-plate and lot-to-lot consistency 100% visual inspection – Ensures each well has proper membrane placement and is free from debris Automation compatible – Manufactured in accordance with SBS guidelines, allowing plates to be run in manual, semi-automated and automated processes
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AcroPrep™ Advance Filter Plates for Ultrafiltration
Pall Life Sciences - Laboratory, Food, Beverage
AcroPrep™ Advance Filter Plates for Ultrafiltration Provides rapid, efficient separation of biomolecules. Omega™ membrane provides high recovery and typically results in > 90% recovery of target biomolecules. New well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume for maximum sample recovery Intrinsic plate and membrane properties prevent target molecules from binding to the plate. Application: Size exclusion, PCR clean-up, Nucleic acid purification, Protein separation.
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AcroPrep™ 24 Filtration System
Pall Life Sciences - Laboratory, Food, Beverage
Eliminates the time-consuming, one-at-a-time syringe filter process by simultaneously filtering up to 24 samples in seconds. Reduces labour time by twenty minutes per carousel over the conventional syringe filter technique. Can be used with any conventional 12 x 32 mm vials; simply transfer your filtered samples from the Waters Alliances sample carousel* to any HPLC sample carousel that houses 12 x 32 mm vials and save both time and labor. No need to use individually wrapped, disposable syringes. Use disposable pipette tips which are less costly, easier-to-use, and require less bench space. Pall's HPLC certification ensures that analytical results will not be compromised by extractable filter material. No cross contamination. Each 1.9 mL well is individually sealed, ensuring your sample will accurately dispense into the Waters Alliance sample carousel with no cross contamination or splashing. Materials of construction are identical to Pall's Acrodisc® syringe filters; if you are currently using Acrodisc syringe filters, this will reduce the requirements for validation of the AcroPrep 24 filter plates. Can be used to process fewer than 24 samples. Simply label used wells or isolate unused wells with parafilm for application at a later time.
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Macrosep® Advance Centrifugal Devices
Pall Life Sciences - Laboratory, Food, Beverage
Quickly concentrate up to 20 mL of biological sample Pall Life Sciences Macrosep Advance Centrifugal Devices Features of the Microsep Advance Centrifugal Devices: • Rapidly concentrates 20 mL sample volumes to 0.5 mL. • Provides high recoveries, typically > 90%. • Low protein-binding Omega membrane and polypropylene housing minimize losses due to non-specific binding. • Versatile Omega membrane is available in a variety of MWCOs. • Built-in deadstop prevents spinning to dryness. • Color-coded for easy identificationApplications of the Microsep Advance Centrifugal Devices: Ultrafiltration • Concentrate and desalt proteins. • Exchange buffer or remove salt of chromatography eluates and gradient fractions. • Recover proteins or other molecules from cell culture supernatants. Microfiltration • Remove particulate from aqueous solutions and clinical samples.
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Microsep™ / Macrosep® Advance Centrifugal Devices
Pall Life Sciences - Laboratory, Food, Beverage
Pall centrifugal devices simplify common nucleic acid and protein handling procedures, replacing traditional separation techniques such as column chromatography, preparative electrophoresis, dialysis, gradient centrifugation, and more. Available in various membrane types to meet your application needs.
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