Optimized cell counting and viability analysis of large, complex A172 glioblastoma cells using the SpectraSlide AP-1 and LUNA-FX7™ automated cell counter

26 Nov 2025

Accurate assessment of cell concentration and viability is vital in cancer research, drug development, and immunotherapy, underpinning the evaluation of experimental outcomes and therapeutic efficacy. However, cell models with large and complex morphologies—such as A172 glioblastoma cells—are challenging to analyze with standard viability assays.

In this study, we compared the SpectraSlide® AP-1 and PhotonSlide™ for cell concentration and viability analysis. Using the A172 glioblastoma cell model, we validated the precision and performance of the SpectraSlide® AP-1.

SpectraSlide® AP-1

Logos Biosystems

The SpectraSlide® AP-1, designed for the LUNA-FX7™ automated cell counter, is an all-in-one cell counting slide that simplifies the sample prep process - saving time & money. No pipettes and no stains needed! Simply load a sample directly on the slide, insert into the LUNA-FX7™ and press COUNT.

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LUNA-FX7™ Automated Cell Counter

Logos Biosystems

The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, new 1, 3 and 8-chamber slide options, state of the art optics, dual fluorescent and brightfield detection, and precision autofocus. The LUNA-FX7™’s new features such as the Bioprocess mode, Quality Control mode, and the CountWire™ (21 CFR Part 11 compliant) software will help companies and facilities meet and surpass quality and regulatory requirements.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.