Optimise Imaging of Growing Cells with Ultra-Flat Glass Bottom Plates

8 Oct 2013
Emily Marquez-Vega
Publishing / Media

Product news

Porvair Sciences new generation Krystal™ glass bottom plates are precision manufactured using a proprietary process resulting in excellent light transmission and an ultra-flat optical plane (+/- 15µm) making them perfect for imaging growing cells.

The complete range of 24-, 96- and 384-well Krystal glass bottom microplates is available sterilised for tissue culture to optimise cell growth or non-sterile for assay development. Deep black plates for fluorescent studies are available, together with brilliant white plates which increase sensitivity for luminescence and absorbance work. The high optical clarity base of each plate in the range offers the advantage of easy positioning and crisp images when used with confocal microscopes. Constructed to a standard SBS / ANSI microplate format - Krystal glass bottom plates are fully compatible with automated liquid handling and robotic devices. All plates are supplied lidded.

In addition to cell growth applications, Krystal glass bottomed plates can be used for light-emitting assays measured through the glass plate base. The special design of the plate virtually eliminates well-to-well light crosstalk inherent with other clear-bottomed microplate designs, enabling unmatched accuracy, higher sensitivity and better repeatability of photometric readings.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.