Olympus Offers Powerful New Software for Confocal Laser Scanning Microscopy Applications

The Olympus ASW v2.0 Software Suite is designed to maximize performance and ease-of-use for the new line of FluoView® FV1000 confocal and FV1000-MPE multiphoton laser scanning microscope systems. The software is designed to enhance experiments in the areas of neuroscience, cell biology, stem cell research, in vivo imaging, electrophysiology, nanotechnology, and retinal imaging, and is also appropriate for core laboratory facilities.

The new software package offers multi-point spatial scanning and stimulation, random mapping scan and pulsed stimulation for maximum performance of experiments using Olympus FV1000 and FV1000-MPE systems. The software streamlines the process of doing multi-area time-lapse experiments.
Multi-point scanning allows high-speed stimulation, imaging or bleaching to occur in multiple spines. The point-to-point speed is under 5 milliseconds, and the accuracy of point location is less than +/- 0.1 micron.

Random mapping scan is an imaging/stimulation method followed by computer reconstruction. No two adjacent, or neighbor, points are ever stimulated sequentially. Random mapping scans are used to reconstruct XY and XYT data out of acquired data. They also help in the reconstruction of electrical activity images. Pulsed stimulation as short as 0.1 millisecond may be applied to uncaging experiments.

For more information on ASW v2.0 software for Olympus multiphoton systems contact Olympus America Inc., 3500 Corporate Parkway, Center Valley PA 18034-0610; phone 1-800-455-8236; visit the company article webpage or email Dennis.Donley@olympus.com.