Nucleofector™ PLUS Supplements Enable Cryopreservation of Cells for Nucleofection™
16 Jul 2012Product news
Lonza introduces Nucleofector™ PLUS Supplements, a new add-on product for use in combination with Nucleofector™ Kits. These specialized supplements are used instead of the standard kit supplement to enable cryopreservation of freshly isolated or harvested cells in Nucleofector™ Solution.
“By decoupling cell harvesting and transfection, Nucleofection™ Experiments can be done at the scientist’s convenience. Cells can be collected, stored and are ready-to-use at a later time, and deliver transfection results comparable to fresh cells. Larger cell batches can be cryopreserved so that researchers can avoid batch-to-batch variances in transfection results. Thus, Nucleofector™ PLUS Supplements can make whole experimental programs more consistent.” explains Dr. Herbert Mueller-Hartmann, Head of R&D Discovery Technologies.
Nucleofection™ is a transfection technology that was first introduced into the market in 2001. It is based on the unique combination of electrical parameters and cell-type specific solutions. It is known as the first efficient non-viral transfection method for primary neural cells and has been stated in over 300 peer-reviewed publications as the method of choice for transfection of primary neurons.
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Lonza Group LtdThe basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format. What benefits are important for your work? Efficient transfection in single cuvette format? Up to 90% efficiency with plasmid DNA Up to 99% efficiency with siRNA duplexes Also suited for peptides, proteins or small molecules Reliable easy-to-use technology? More than 150 ready-to-use Amaxa® Optimized Protocols High viability and preservation of cell funcionality Data for more than 650 cell types in our Cell Database Consumables tailored to your needs? Nucleofector® Kits are available in low (10 reactions), normal (25 reactions) and high (100 reactions) usage format More than 50 dedicated primary cell kits available A collection of 5 cell line kits and an optimization kit covering the broadest range of cell lines cGMP kits available
The Nucleofector Device
Lonza Group LtdThe Nucleofector technology is a highly efficient non-viral method for transfection. It is based on two components: the Nucleofector Device that delivers unique electrical parameters, and Nucleofector Kits that contain cell-type-specific Nucleofector Solutions. The electrical parameters of the Nucleofector are different from any other commercially available electroporation device. Each electrical setting is displayed as a distinct program which has been adapted to the requirements of a particular cell type. As the electrical settings are pre-programmed into the Nucleofector, optimization of the electrical parameters by the user is not necessary. The device weighs only 2.6 kg and is conveniently sized so that it easily fits under a sterile hood, which greatly facilitates sterile work with cells.