NucleoBond<sup>®</sup> Xtra - the faster anion-exchanger now available for purification of endotoxin-free plasmid DNA

17 Jul 2008
Greg Smith
Analyst / Analytical Chemist

Product news

Based on the innovative NucleoBond® Xtra product line MACHEREY-NAGEL has developed the endo-free version for ultra-fast plasmid preps with higher yields of endotoxin-free DNA: NucleoBond® Xtra EF.

High-speed lysate filtration and increased column flow rates lead to up to 68% reduced prep times. Improved binding capacity of the columns and optimized buffer compositions lead to 100% increased yields compared to other commercially available kits for endotoxin-free plasmid purification. NucleoBond® Xtra EF is available in Midi (> 250 µg DNA) and Maxi (> 1000 µg DNA) format. The ultra-fast Plus versions include NucleoBond® Finalizers, a desalting tool for speeding-up final DNA precipitation.

DNA with less than 0.05 EU/µg allows transfection of even highly sensitive cell lines (e.g. primary neurons).

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.FiltrationFiltration and microfiltration are used to separate solids, particulates or large molecules from fluids (liquid or gases) in order to purify the filtrate. Explore a range of filter formats, including filter paper, plates and membranes for your separation needs. While larger particulates may be separated by gravity filtration, smaller particulates may require additional force for timely separation. Microfiltration equipment includes vacuum filters, as well as positive pressure, crossflow and centrifugal filtration devices. Find the best filtration products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.