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Novel Affinity Purification Process for Camelid Antibodies

KNAUER Wissenschaftliche Geräte GmbH
8 Nov 2016
Emily Adam
Publishing / Media

Industry news

KNAUER Wissenschaftliche Geräte GmbH and 3B Pharmaceuticals GmbH have announced the successful completion of a federal grant program exploiting the potential of camelid antibodies.

Combining 3B Pharmaceuticals’ PurAffin™ platform for affinity ligand design and KNAUER’s expertise in chromatography systems and matrix development, the companies delivered a purification process for camelid heavy chain antibodies to the project consortium. Due to their comparatively low molecular weight, high stability and better bioavailability, heavy-chain antibodies have shown great potential as a resource for the development of therapeutics and research tools.

“KNAUER’s expertise in downstream-process development and automated continuous chromatography perfectly complements 3B Pharmaceuticals PurAffin™ platform”, states Dr. Matthias Paschke, Director Biology & Assay Technologies at 3B Pharmaceuticals. “Camelid antibodies are the latest class of biologics for which peptidomimetic affinity purification solutions have become available.”

“Through the excellent collaboration with 3B Pharmaceuticals we are proud to reach yet another milestone in our purification service portfolio at KNAUER´s LC LAB department” added Dr. Veit Althoff, project leader and application specialist at KNAUER. “The new Azura Bio systems perfectly overcame the innovative challenge of a scalable camelid antibody purification process”.

The consortium comprising five project partners from industry and academia is led by Prof. Katja Hanack from Potsdam University. The “Camelid antibody” project has received funding from the Federal Ministry of Education and Research within the framework of the BMBF - innovation initiative “InnoProfile-Transfer” supporting mid-sized companies in the Berlin-Brandenburg region under grant agreement 03IPT703.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Antibody PurificationMolecular DesignChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.PeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.Matrix Effects

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Novel Affinity Purification Process for Camelid Antibodies