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NEXTflex Small RNA Sequencing Kit for NGS Library Prep Launched

4 May 2011
bridget bridget
Laboratory Director

Product news

Bioo Scientific launched the patent pending NEXTflex™ Small RNA Sequencing Kit which provides an easy, flexible, cost-effective solution for generating single and multiplexed libraries from small RNA using Illumina GAIIx and HiSeq 2000 sequencing platforms. The NEXTflex Small RNA Sequencing Kit allows users to generate libraries directly from as little as 1 μg of total RNA.

This kit contains a specialized 3’ adenylated adapter that specifically ligates onto microRNAs and other small RNAs containing a 3’ hydroxyl group. After adapter ligation and reverse transcription, libraries are amplified using a barcoded primer, allowing up to 48 samples to be multiplexed and sequence variations interrogated. The NEXTflex Small RNA Sequencing Kit incorporates AIR™ Ligase, a patent pending, truncated T4 RNA Ligase, which significantly increases the efficiency with which small RNAs are tagged with adapter, giving greater sequence depth. The new protocol is designed to significantly reduce adapter dimer formation prevalent in other protocols.

Strand specificity is retained through the use of adapters that ligate to the 5’‐phosphate and 3’‐hydroxyl groups that most mature miRNAs possess. The kit can be used for mRNA directional sequencing. In combination with the NEXTflex™ Small RNA Barcode Primer kits, 48 samples can be profiled and studied simultaneously. Four sets of 12 NEXTflex™ Small RNA Barcode Primers, with embedded index sequences are available offering an improved multiplexing workflow and increased flexibility. This new automation-friendly format enables multiplexing of up to 384 samples.

The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. The primer barcoding system utilizes a 6 nt index to differentiate up to 48 different samples on a single flow cell lane. In addition to small RNA sequencing, the NEXTflex Small RNA Sequencing Kit is compatible with mRNA directional, RIP-Seq and CLIP-Seq protocols.

The NEXTflex Small RNA Sequencing Kit and NEXTflex Small RNA Barcode Primers are the latest additions to Bioo Scientific’s portfolio of next generation sequencing solutions. These kits demonstrate Bioo Scientific’s commitment to developing innovative solutions to improve, simplify and reduce the costs of next generation sequencing.

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