Next Generation Sequencing: The Power of Two – Ribosomal RNA Removal and Directional Library Generation in One Package

12 Nov 2012

Product news

Cambio, the exclusive distributors of Epicentre products in the UK and Ireland, have added a new range of products to their portfolio. The ScriptSeq™ Complete kits seamlessly combine Epicentre’s powerful Ribo-Zero™ technology and rapid ScriptSeq™ v2 procedure for end-to-end preparation of RNA Sequence libraries in just one day.

The kits enable life scientists to quickly produce directional, paired-end RNA-Seq libraries, typically containing less than 3% rRNA reads, from as little as 100 ng total RNA. 48 ScriptSeq™ Index PCR Primers are available separately for preparing barcoded libraries. Until the 31 December 2012, researchers can get a free ScriptSeq™ Index PCR Primer set with the purchase of every 24 reaction ScriptSeq™ Complete kit. ScriptSeq™ Complete kits are currently available for human, mouse and rat samples, but Complete kits for bacteria and plants are expected in early 2013.

ScriptSeq™ Complete kits remove the need to purchase separate kits for the different steps of RNA-Seq library generation, thereby reducing the risk of possible sample loss, hold-ups and unexpected costs. The Complete kits have been designed for a rapid, simple workflow, and are less expensive than buying the components separately. Cambio is dedicated to providing UK laboratories with the most efficient and effective tools for life science applications. Fully supported by top quality, tailored customer service, users are supplied with a complete solution that allows them to see the entire RNA-seq process through from start to finish.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library GenerationLibrary generation refers to the construction of NGS libraries from RNA and DNA sources.RNA-SeqRNA sequencing (RNA-seq) is a next-generation sequencing technique used to analyze the transcriptome, providing insights into gene expression and regulation. This method is essential for genomics, disease research, and personalized medicine. Explore RNA-seq tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.