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Next-Generation Large Molecule Affinity Purification Solution Improves Biotherapeutic, Disease Biomarker and Sports Anti-Doping Bioanalysis

Thermo Fisher Scientific

Thermo Scientific MSIA Streptavidin EVO microcolumns simplify large molecule bioanalysis

31 Mar 2016
Lynsey Forsyth
Post Doc / Research Fellow

Product news

A new fully automated affinity purification solution is designed to enable scientists to analyze large biomolecules, such as proteins and antibodies, with improved accuracy, consistency and simplicity, even at high throughputs. The new Thermo Scientific MSIA Streptavidin EVO microcolumns have been designed to bring the novel mass spectrometric immunoassay (MSIA) affinity purification technology to Tecan's Freedon EVO® series of robotic platforms, that are equipped with a 96 multichannel liquid arm (MCA96).

When combined with the Tecan Freedom EVO robotic platform, MSIA Streptavidin EVO microcolumns enable fast and accurate isolation of target analytes from complex biological matrices. Housed within a pipette tip, the proprietary monolithic columns are densely coated with streptavidin for analytical affinity purification of any biotinylated affinity ligand, even at low concentration. Since this technology is incorporated into a pipette tip, users can analyze sample volumes as low as 10 µL, saving precious samples. Furthermore, when compared to traditional resin and bead-based methods, MSIA technology provides lower background noise and improved analytical sensitivity, while the simple workflow enables easier method standardization and transfer between labs.

“The MSIA Streptavidin EVO microcolumn is an ideal solution for biologics and biosimilars discovery and development,” said Preeti Saini, product manager for MSIA liquid handling consumables at Thermo Fisher Scientific. “It is particularly well suited to laboratories performing large molecule bioanalyses that demand high-quality, reproducible and unambiguous results with minimal background noise. Moreover, this solution is designed to help scientists save both precious samples and time.”

“Our combined solution will provide these laboratories with faster assay development, scalable sample processing and surety of data quality and consistency,” added Rohit Shroff, product manager for Tecan. “By bringing together Tecan’s well-established Freedom EVO workstations and Thermo Scientific MSIA technology, we can better cater to the high-volume bioanalytical research market.”

Until now, the MSIA technology has been exclusively available for use with the Thermo Scientific Versette Automated Liquid Handler and Thermo Scientific Finnpipette Novus i Multichannel Electronic Pipette for high- and low-throughput applications, respectively. The MSIA Streptavidin EVO microcolumns have been designed to enable a broader community of high-throughput bioanalytical researchers to benefit from automated liquid handling for large molecule bioanalytical assay development.

Visit our website for more information on the MSIA technology and how MSIA workflow solutions enable highly reproducible and sensitive testing of large molecule analysis.

Visit the Tecan website for more information on Tecan’s robotic liquid handling solutions.

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MSIA™ Microcolumns for the Freedom EVO Platform

Thermo Fisher Scientific

Next generation large molecule bioanalysis made easier by combining the Thermo Scientific™ innovative MSIA™ technology with Tecan advanced, robust and easy-to-use liquid handling. MSIA affinity microcolumns, designed for Freedom EVO platform (MCA 96 head), provide innovative enablement in the areas ranging from bioanalysis of therapeutic mAbs, antibody-drug conjugates (ADC), biotransformation [in-vivo modifications, drug-antibody ratio (DAR)], preclinical and clinical drug trials (PKPD, DMPK, and ADME) to biomarker and proteins analysis. This unique solution is designed to deliver the accuracy, reliability and automation you need to be successful.   Features: Robust Automation:  Simply program the Freedom EVO to elute the analyte from complex biological samples and let the automated platform carry the load. Highly sensitive: Analyze proteins of interest down to femtomole level. Enhanced characteristics:  Detects multiple protein variants for qualitative and quantitative bioanalysis in a single assay. Contaminant-free: proprietary molecular trapping microcolumn technology prevents matrix injection that would impact LC-MS function. High signal-to-noise ratio: Innovative sample interface technology reduces matrix loss, reagent carryover and non-specific binding. Time Savings: Microfluidic channels drive kinetic interactions significantly decreasing incubation time required to process the samples, while the MCA 96 head enables parallel processing of 96 samples. Reproducible: Consistent performance for assay reproducibility. Preserving samples: Small sample volumes (as low as 10μL) and a reduced requirement for repeating experiments. MSIA Streptavidin EVO microcolumns provide end user flexibility to analyze their specific targets of interest.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. 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Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. 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Important considerations of biopharmaceutical use include application, cost, production process and purification.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. 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Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.PurificationPurification techniques are essential for isolating compounds of interest from complex mixtures, such as proteins, nucleic acids, and chemicals. These techniques are critical in biochemistry, drug development, and environmental studies. Explore purification systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. 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