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Nexera MP UHPLC Front End System for LC/MS: LC/MS Analysis in Drug Detection Processes

7 Jul 2011
bridget bridget
Laboratory Director

Product news

Shimadzu, one of the worldwide leading manufacturers in analytical instrumentation, has introduced the Nexera MP UHPLC as a Front End system for LC/MS. The system premiered in Europe at the HPLC 2011 show in Budapest, Hungary, from June 19 to 23. The Nexera MP is perfect for LC/MS analysis conducted in pharmacokinetics and synthesis stages in drug detection processes.

The new SIL-30ACMP Multiplate Autosampler used in the Nexera MP is best suited for LC/MS analysis and has the industry-best micro-volume injection repeatability, the world’s highest injection speed and the lowest carryover. Combining the Nexera MP with Shimadzu’s LCMS-8030 Triple Quadrupole Mass Spectrometer or the LCMS-2020 Single Quadrupole Mass Spectrometer creates a system for quick analysis of multiple samples with high accuracy.

Development Background
Pharmacokinetics and candidate compound synthesis stages in drug discovery processes need fast analysis of multiple samples using an LC/MS system with high throughput. Minimization of carryover in samples is also important for enhancement of LC/MS analysis reliability, since mass spectrometers are becoming more and more sensitive.

The synthesis stage demands a system where two or more users can share an instrument and measure samples without delay, so that candidate compounds can be checked and identified quickly for more efficient analysis. This setup also reduces power consumption, thereby lessening the burden on the environment.

Combined with high-performance accessories, the Nexera MP UHPLC Front End system achieves outstanding features:

• Efficient sample preparation
The SIL-30ACMP Multiplate Autosampler provides excellent injection repeatability with micro volume injections (e.g. 1% max. in 0.5 µL injections). The influence of sample solvents, which causes problems in analysis of deproteinized or synthetic samples, is minimized by reducing the injection volume. Samples can therefore be injected directly without dilution, streamlining the preparation process.

• Multi-sample processing with the world's fastest injection
In addition to fast separation of components, the Nexera MP uses the SIL-30ACMP Multiplate Autosampler featuring the world’s fastest injection at just 7 seconds which enables ultra-high-speed analysis cycles. In addition, up to 2304 samples can be set. Ultra-high-speed multi-sample processing is enabled, necessary in pharmacokinetics and synthesis confirmation processes. Ultra-high-speed analysis reduces consumption of both power and organic solvents used for analysis, reducing in turn the environmental burden.

• Industry-lowest level carryover
The Nexera MP provides ultra-low carryover, essential for LC/MS analysis. By incorporating the exceptional technology used in the Shimadzu prominence / Nexera Series autosamplers to effectively prohibit adsorption in the sample line, the SIL-30ACMP achieves an industry-lowest level carryover of 0.0015 % max. (no rinse). A multi-rinse function allows selection of the optimum rinse method depending on the sample. These functions ensure reliable analysis even when a high-sensitivity mass spectrometer is used.

• Two or more analyzers share one system
Two or more experimenters often share one system in the compound confirmation process. Using the Nexera MP, each can set a sample plate without delay, even during analysis. Analyses previously run in multiple systems can therefore be run on one system, saving energy and operation costs.

• Column oven setting to the MS position
To achieve ultra-high-speed analysis using LC/MS, the length of the piping connecting the HPLC and mass spectrometer needs to be minimized in order to fully exploit the separation performance. When mounting the optional CTO-30AS Column Oven to the autosampler, mounting height can be adjusted in three steps and the angle can be adjusted flexibly. As a result, the column exit can be positioned according to the height of the LC/MS interface, helping to realize ultra-high-speed, high-separation LC/MS analysis while maximizing the system’s separation performance.

The Nexera MP is controlled by Shimadzu’s LabSolutions software and major LC/MS workstations available from other companies.

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AutosamplersAutosamplers are automated sample loaders used with a number of separation and analytical techniques. HPLC autosamplers use automated partial or full-loop injection systems and place samples in vials or microplates for analysis. Autosamplers can also be used for elemental analysis. Features to consider include repeatability, syringe capacity, footprint and temperature controls.  UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Combinatorial ChemistryCombinatorial chemistry, also known as combichem, is a technique used in drug discovery to create libraries of structurally related compounds. A library is generated by synthesis with a chemical reactor system or by computer-based modeling of compound combinations. When undertaking combinatorial chemistry consider reagents, buffers, resins and standards.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.Medicinal ChemistryMedicinal chemistry is a broad discipline encompassing the design, identification, synthesis and development of chemicals in drug discovery. It includes a number of techniques covering structural biology, synthetic chemistry and molecular biology. Technologies used in medicinal chemistry include ADME, lab-on-a-chip, high content screening and assay assembly.Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.

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