New Water Immersion Objective Designed For Multiphoton Imaging

Olympus has introduced a new 25x water immersion objective specifically designed for multiphoton imaging. It offers researchers outstanding deep-imaging and high resolution performance with the Olympus FluoView FV1000MPE multiphoton laser-scanning microscope system.

The new Olympus XLPlan N 25x objective has been designed with a numerical aperture (NA) of 1.05 for high-resolution multiphoton microscopy. It can be used for tissue samples with cover slips and for investigations, such as patch clamping, where the sample cannot be covered. As a result, higher z-resolution and significantly increased brightness is achieved for all deep imaging applications, compared to similar objectives already on the market.

With a working distance of 2 mm and a 35-degree angle available for patch clamping, this objective is excellent for neurophysiology and related applications. In addition, a super-wide field of view (27.5) aids researchers in obtaining more information from each multiphoton experiment. Furthermore, a correction collar compensates for refractive index mismatches, allowing excellent axial and spatial resolution hundreds of microns into living brain samples.

This is the first time ever that a life science objective has been dedicated to this fast growing imaging area. As a result it has been designed to maximise the effectiveness of the multi-photon process, with chromatic aberrations corrected within Ti:Sa IR-laser wavelength range 680-1100 nm. In addition, new optical coatings allow the objective to provide outstanding performance in the IR range, with permanent transmittance of more than 82% from 400 to 1000 nm. This results in at least 5x the normal brightness levels during multi-photon excitation, combined with more efficient collection of emitted light, providing greatly enhanced instrument sensitivity and higher signal-to-noise ratios.

The new Olympus XLPlan N 25x (N.A.1.05) objective is designed specifically for deep-specimen imaging, delivering the maximum detection efficiency.