New Toyopearl Q-600C AR (alkaline resistant) Anion Exchange Resin

Tosoh Bioscience introduces the new Toyopearl Q-600C AR ion exchange resin. It is well-suited for high capacity capture of biotherapeutics or plasma proteins. ‘AR’ stands for alkaline resistance as the resin combines high dynamic binding capacity and fast mass transfer kinetics with an improved alkaline stability. Thus, Toyopearl Q-600C AR is enabling both, high process throughput and improved process robustness.

Ion exchange chromatography is frequently used as capture or intermediate step in the purification of proteins. The development of cell lines with high expression levels has triggered the demand for high throughput in downstream processing. Increasing throughput requires rigid resins with high binding capacity withstanding high flow rates and harsh purification procedures. The new Toyopearl Q-600C AR capture resin with an average particle size of 100 µm was designed to meet these requirements.

Based on the well proven methacrylic polymer backbone of Toyopearl and TSKgel media Toyopearl Q-600C AR provides excellent pressure flow characteristics. It has a slightly larger pore diameter than the well-established Toyopearl QAE-550C resin, but its ligand attachment chemistry creates significantly more dynamic binding capacities of > 100 mg BSA and 90 mg IgG per mL resin. It tolerates caustic cleaning solutions up to 1.0 mol/L NaOH well with essentially no change in DBC. Toyopearl Q-600C AR is therefore ideally suited for process scale protein purification in large industrial process columns.