New System for Fast and Consistent Results Makes UHPLC Widely Accessible

Thermo Scientific Vanquish Flex Binary UHPLC system boosts high-speed applications and provides exceptional LC and LC-MS performance

20 Jun 2016
Weylan Kiam-Laine
Microbiologist

Product news

Researchers looking for fast and consistent results can now benefit from the new ultra high-performance liquid chromatography (UHPLC) system from Thermo Fisher Scientific to boost their LC and LC-MS performance.

As the latest addition to the Thermo Scientific Vanquish UHPLC platform, the new Thermo Scientific Vanquish Flex Binary UHPLC system adds a binary solvent delivery option in the 1000 bar (15,000 psi) performance range. The system is built for high-speed, fast gradient applications and the Vanquish platform’s inherent outstanding retention time reproducibility. The Vanquish Flex Binary UHPLC system is the solution for laboratories performing qualitative and quantitative LC or LC-MS analysis that need to increase sample throughput. Ease-of-use and innovative technologies that are robust by design are making UHPLC widely accessible and enable easy and effective method transfer from established systems and applications.

“As the newest addition to our Vanquish platform, we developed this system with speed, sensitivity and quality results in mind—providing the level of operational efficiency required by today’s researchers,” said Fabrizio Moltoni, vice president and general manager, HPLC at Thermo Fisher. “The Vanquish Flex Binary system showcases these attributes and provides exceptional LC and LC-MS performance together with our products for sample preparation, reagents, columns, mass spectrometry and data processing.”

The Vanquish Flex Binary System features a binary high-pressure gradient pump with 2 x 3 solvent channels and low gradient delay volume capable of delivering high flow rates of up to 8 mL/min. The entire system provides biocompatible flow path, SmartInject using intelligent sample pre-compression, selectable thermostatting modes and a unique range of detection capabilities.

The Vanquish Flex Binary system will be exhibited at the 44th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC) in the Thermo Fisher Booth #601 from June 19-24 at the Marriott Marquis in San Francisco.

Thermo Scientific™ Vanquish™ Flex Quaternary UHPLC System

Thermo Fisher Scientific

The Thermo Scientific™ Vanquish™ Flex Binary UHPLC system is biocompatible with a state-of-the-art binary high-pressure pump and state-of-the-art binary solvent blending. This ultra high performance liquid chromatography system shares all Vanquish values, such as a design focused on uptime, robustness and reliability. Multiple detection options as well as Thermo Scientific™ Viper™ fingertight connections throughout the system will give you the performance you need with the ease of use you want. Our SmartFlow™ pumping technology with automatic compensation for changing eluent compressibility ensures excellent flow and gradient precision, independent of eluent composition and backpressures. This gives you unrivaled retention time precision and highest data confidence, even for ternary and quaternary gradients, and for more freedom in method development and application switching. Detection capabilities include high sensitivity and linearity Diode Array Detection with LightPipe technology, selective and sensitive Fluorescence Detection and near-universal detection capabilities with the Vanquish Charged Aerosol Detector. Key Features: Integrated modularity design concept to offer uncompromising UHPLC State-of-the-art quaternary solvent blending 1000 bar of pump pressure and flow rates up to 8 mL/min Industry-leading flow and gradient precision Multiple detection options including DAD, FLD and CAD Relevant Applications: Separations of Intact mAbs Analysis of labeled glycans Ternary gradient impurity profiling Efficient, reliable peptide mapping

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Quantitative AnalysesQuantitative analyses involve measuring the amount or concentration of a substance in a sample using analytical techniques. These analyses are essential in fields like chemistry, biology, and environmental science. Explore quantitative analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Sample PreparationHPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.LC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.
New System for Fast and Consistent Results Makes UHPLC Widely Accessible