New Stripping Buffers for Efficient Reprobing of Western Blots

12 Oct 2014
Sarah Thomas
Associate Editor

Product news

LI-COR now offers two new products for stripping and reprobing of Western blots for qualitative analysis.

WesternSure™ ECL Stripping Buffer provides a high-quality solution to effectively strip both primary and secondary antibodies from Chemiluminescent Western Blots and is the latest addition to the WesternSure product family. It can be used with PVDF and nitrocellulose membranes.

NewBlot™ IR Stripping Buffer is a cost-effective formulation for stripping and reprobing near-infrared (NIR) Western blots. An addition to the existing NewBlot Stripping Buffers, NewBlot IR removes both primary and secondary antibodies and has lower shipping costs compared to the existing products. It is also suitable for use with both membrane types.

"Both WesternSure ECL and NewBlot IR Stripping Buffers maintain target antigen integrity for efficient reprobing of blots," says Shawn Mischnick, technical product manager at LI-COR Biosciences. "These high quality products will provide customers an effective and economical alternative to homemade stripping buffers."

WesternSure ECL Stripping Buffer

LICORbio

WesternSure ECL Stripping Buffer provides a robust solution for stripping primary and secondary antibodies from Western blot membranes while maintaining target antigen integrity for efficient reprobing.

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NewBlot™ IR Stripping Buffer

LICORbio

NewBlot IR Stripping Buffer is ideal for stripping and reprobing nitrocellulose and PVDF membranes detected with IRDye ® secondary antibodies. It may also be suitable for other dye-conjugated secondary antibodies.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Blotting