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New Standalone Ultrahigh-Pressure Pump for LC Applications

Thermo Fisher Scientific
28 Jun 2010
Sarah Sarah
Marketing / Sales

Product news

The new UltiMate® 3000 NCP-3200RS nano/capillary LC pump module from Dionex Corporation is an extension of its family of industry-leading Rapid Separation LC (RSLC) systems. This standalone ultrahigh-pressure pump features an extensive flow-pressure footprint, delivering continuous, direct flow rates from 20 nL/min to 50 μL/min at backpressures of up to 800 bar (11,600 psi) for nano, capillary, and micro LC analyses.

The pump can be used to configure an entry-level RSLCnano system for reversed-phase separation of peptides in LC-MS proteomics workflows. Alternatively, the pump can be used as an add-on module to our industry-leading RSLCnano system for advanced applications, such as automated off-line 2D-LC or tandem nano LC. Tandem nano LC can be an attractive technique for customers who want to improve their mass spectrometry use and increase sample throughput. Dedicated application kits have been introduced for the RSLCnano system, such as automated off-line RP × RP of peptides and tandem nano LC, to facilitate instrument installation and get effective data from the first experiments.

In addition, Dionex Corporation has also released new biocompatible switching valves, featuring a maximum pressure specification of 345 bar (5,000 psi), for sample injection and column switching. These values are intended for use with the RSLCnano system, to provide a biocompatible sample flow path that can be important to customers dealing with the analysis of phosphopeptides.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.PumpsLaboratory pumps are devices used to move liquids and gases by vacuum or positive displacement. The pressures/vacuums generated are determined by the type of pump used. High vacuum pumps include diffusion, ion, roots and turbopumps; for primary/medium vacuum applications see rotary vane pumps, scroll & diaphragm pumps. For the positive displacement of liquids explore a range of syringe and peristaltic pumps, as well as fluid aspirators. Discover the best pumps in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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