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New SageHLS Purifies Large Fragments of DNA for Long-Range Genomics

Sage Science
14 Feb 2017
Mia Harley
Biochemist

Product news

Sage Science, a developer of products for improving sample preparation processes in life science applications, have announced the launch of a new platform that can extract and purify extremely large DNA fragments directly from bacterial and tissue cultures, blood samples, or other cell sources. Large DNA fragments are required for long-range sequencing, optical mapping, droplet digital PCR, and more. The SageHLS platform will be on display at the Advances in Genome Biology and Technology (AGBT) meeting in Hollywood, Fla., this week.

Many genomic applications work best with high molecular weight (HMW) DNA, or very large DNA fragments. However, traditional sample preparation processes cannot manage DNA in these sizes, hindering scientists’ ability to capture optimal information from long-read and long-range sequencing, optical mapping, droplet digital PCR, and single-molecule tools. The SageHLS platform (short for HMW Library System) can purify DNA into fragments ranging from 50 kilobases to 2 megabases, providing the high-quality, long fragments needed for resolving haplotypes, structural variants, and other large or linked genomic elements.

“We believe that access to DNA molecules hundreds of kilobases or even megabases long will allow scientists to embrace advanced genomic technologies and reveal new insights about how large DNA elements influence disease and other phenotypes,” said Todd Barbera, Chief Executive Officer of Sage Science. “The SageHLS platform can purify DNA in sizes that no other tool can consistently handle.”

In beta testing, scientists at academic medical institutions have used the SageHLS instrument to extract DNA as long as 2 Mb as well as to perform targeted purification of genomic fragments with CRISPR/Cas9. Additional applications for the system are already under development.

For most samples, the SageHLS can recover at least a microgram of DNA, a sufficient volume for common genome applications. At launch, users will perform traditional library prep after purification on the SageHLS platform, but later versions of the system will directly incorporate this step for a more streamlined workflow.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. 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Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Droplet Digital PCRDroplet digital PCR (ddPCR) is a method of quantitative PCR that partitions a sample into droplets, allowing precise detection of low-abundance targets. It is commonly used in gene expression analysis and mutation detection. Explore ddPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Genome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Gene EditingGene editing is the precise alteration to an organism's DNA, with CRISPR being the most well-known technique. It is used in a wide range of applications, including the development of disease models and gene therapy, and crop improvement. Browse our peer-reviewed product directory to find the best gene editing solutions, compare products, check reviews, and get pricing directly from manufacturers.CRISPRCRISPR technology enables precise editing of genes, allowing scientists to modify DNA at specific locations. This revolutionary tool is used in genetic research, drug development, and gene therapy. CRISPR has applications in agriculture, disease treatment, and creating genetically modified organisms (GMOs). 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New SageHLS Purifies Large Fragments of DNA for Long-Range Genomics