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New SageELF Technology Generates Multiple Fractions from a Single DNA or Protein Sample

Sage Science
14 Feb 2014
Sarah Thomas
Associate Editor

Product news

Sage Science, a developer of products for improving sample preparation processes in life science applications, has announced the release of its new SageELF technology for DNA and protein fractionation. This sample prep instrument will be especially useful for preserving precious samples and has applications in next-generation sequencing as well as bottom-up or top-down proteomics experiments.

The SageELF generates 12 contiguous fractions from whole DNA or protein samples. For DNA or cDNA sequencing, that allows scientists to generate libraries with multiple insert sizes from the same sample, which has applications in genome sequencing, structural variant detection, and identification of rare splice variants. For protein studies, the ability to take many slices from a whole sample is useful for reducing sample complexity, enriching for rare proteins, and ensuring recovery of all proteins in the sample.

Maximize the Value of Every Sample

"As the leading provider of automated products for targeted DNA size selection, we are pleased to offer this new tool for whole-sample fractionation. We believe that users will find the SageELF to be more reproducible and precise than manual gels or columns," said Todd Barbera, Chief Operating Officer of Sage Science. "The ability to maximize value from every single sample is in line with our commitment to the sample prep market, where we target labor-intensive steps that can be significantly improved with accurate, automated solutions."

Simple Operation

The SageELF, short for Electrophoretic Lateral Fractionator, automatically electrophoreses samples and elutes whole DNA or proteins from the entire gel lane into 12 elution modules. Users simply load their sample, start the run, and pipette out the fractions later.

Unlike in-gel digestion, in which some DNA fragments or peptides never leave the gel, the whole protein or DNA sample is electrophoresed into the elution modules.

Optimize Sequencing Efficiency

Sage Science also manufactures the Pippin line of automated tools for targeted DNA size selection. Independent studies of size-selection methods for next-generation sequencing sample prep have repeatedly found that the Pippin platform offers unparalleled reproducibility, accuracy, and sample recovery. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing project costs.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.

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New SageELF Technology Generates Multiple Fractions from a Single DNA or Protein Sample