SelectScience
Join Free
Accelerating Science
NewsLife Sciences

New RT-PCR Assay Kit for Simultaneous Detection of Zika, Dengue, and Chikungunya Viruses

Bio-Rad
14 Jun 2017
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

Bio-Rad Laboratories, Inc. today announced the launch of an RT-PCR kit that allows researchers to simultaneously screen samples for Zika (ZIKV), dengue (DENV1, DENV2, DENV3, DENV4), and chikungunya (CHIKV) arbovirus RNAs. The assay comes with all the controls researchers need to internally validate the results. This assay is approved for Research Use Only and is currently available only outside the U.S.

The ZDC Multiplex RT-PCR Assay (Research Use Only) is a one-step reverse-transcription, real-time PCR assay for the simultaneous detection and quantification of ZIKV, DENV, and CHIKV RNAs from a large range of specimen types.

“Our assay provides researchers an effective and reliable tool for the concurrent detection of Zika, dengue, and chikungunya RNAs in a single reaction tube,” said Luca Boveri, PhD, Bio-Rad Global Product Manager, Gene Expression. “Researchers can now rely on a wet-lab validated tool whose key components have been manufactured in GMP-approved facilities and extensively tested to generate consistent results.”

Independent screening for Zika, dengue, and chikungunya can be time-consuming and challenging, particularly when the number of samples is sizable or the amount of starting material is limited, as is often the case when dealing with serum, blood, or even mosquitoes. By taking advantage of a multiplex setup that includes the capability to detect all three viral targets and up to two different controls in a single tube, Bio-Rad’s assay delivers a sensitive tool that maintains excellent linearity and efficiency while also providing a streamlined workflow.

Bio-Rad has validated its assay on popular real-time PCR platforms, thus assuring investigators of consistency across different instruments. Bio-Rad rigorously evaluated and optimized the assay for specificity, sensitivity, reproducibility, and efficiency.

Other Key Benefits

  • One-step RT-PCR protocol for a streamlined workflow
  • Up to 5-plex capability, including viral targets and controls
  • Kit contains all components needed to run 200 reactions

Links

Bio-RadCompany website

Tags

Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Screening SystemVirusesViruses are microscopic pathogens that require a host cell to replicate. Understanding their structure, replication cycle, and impact on the immune system is crucial in developing effective treatments, vaccines, and diagnostic methods. Research continues to focus on emerging viral diseases and antiviral drug discovery. Explore solutions for virus research, detection, and treatment in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.MicrobiologyRT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ZikaInfectious DiseasesInfectious diseases are caused by pathogens such as bacteria, viruses, and fungi. Effective detection, prevention, and treatment are crucial to control outbreaks and improve public health. Diagnostic tools, vaccines, and antimicrobial treatments are essential components of managing these diseases. Research into emerging pathogens and resistance patterns continues to evolve. Browse our peer-reviewed product directory to find the best diagnostic tools, prevention solutions, and treatment options; compare products, read customer reviews, and get pricing directly from manufacturers.

Related articles