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New Promega QuantiFluor RNA System for Sensitive, Easy Quantitation

Promega Corp.
23 Feb 2012

Product news

Promega Corporation announces the launch of QuantiFluor™ RNA System for highly sensitive, easy RNA quantitation. The QuantiFluor RNA dye enables quantitation of small amounts (as little as 100pg) of RNA in solution; a critical step in many biological applications, particularly in standard molecular biology techniques.

The new system provides significantly increased sensitivity for those samples that are low in concentration, using far less template RNA to accurately quantitate compared with alternative systems, and with better linearity and detection limits.

The QuantiFluor RNA System includes all required reagents to quickly set up and quantitate RNA, offering an integrated solution with instrument pairing. The System is easy to set up on numerous platforms including both the QuantiFluor™ and GloMax® Multi instruments.

Applications include measuring yields of in vitro transcribed RNA, and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, RT-PCR, and differential display PC.

The new dye system is the latest addition to Promega’s nucleic acid quantitation dyes, which also includes QuantiFluor dsDNA System.

Please follow the link below for more information.

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QuantiFluor™ RNA System

Promega Corp.

  The QuantiFluor™ RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is a very important step used in many biological applications, particularly in molecular biology techniques. These include measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation and reverse transcription PCR. Features - Benefits Increase your Sensitivity: Significantly increased sensitivity over absorbance at 260nm (NanoDrop®) for those samples that are low in concentration. Save Precious Sample for Downstream Assays: Less template RNA required than spectrophotometry. Setup Quickly and Easily: System includes all the necessary reagents to quickly set up and quantitate RNA. Experience Flexible Instrument Compatibility: Sets up easily on both the QuantiFluor™ and GloMax®-Multi Instrument lines. This system also can be used on any fluorescent instrument with appropriate optical channels. Remain Cost-Effective: Value priced for those customers who are cost-conscious and budget-constrained. Applications RNA quantitation. Notes Each QuantiFluor™ RNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl.  

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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New Promega QuantiFluor RNA System for Sensitive, Easy Quantitation