New Product Line for Advanced Colony Counting in Quality Assurance Labs of the Pharmaceutical Industry

15 Mar 2011
bridget bridget
Laboratory Director

Product news

Sartorius Stedim Biotech, a leading supplier of products and services for the biopharmaceutical industry, is expanding its portfolio for quality assurance in the lab. Microsart® @vance® is the new product line that joins the established Microsart® family, and has been specifically designed for microbiological quality control in the pharmaceutical and biopharmaceutical industries.

Microsart® @vance® enables efficient, advanced work flows in quality assurance laboratories and meets the highest safety standards. As a result, the new product line more than meets the demanding requirements of the pharmaceutical and biopharmaceutical industries. The single-use filter units, Microsart® @filter 100 and 250, lead the vanguard of this new range and have been specifically developed for the detection and enumeration of microorganisms in pharmaceuticals and cosmetics.

Efficient Analysis, Reliable Results

Just a few steps are all it takes to go from sampling to incubation using Microsart® @filter and obtain reliable analytical results. The filter units are sterile-packaged, ready to connect and can be used right “out of the box” in the quality assurance laboratory. Thanks to the optimized design of the filter unit, the entire sample is filtered, without any liquid retained on the funnel walls. This eliminates the need for rinsing after filtration, unless required by specific regulations.

The filter and funnel form an all-in-one, sterile unit and thus minimize the risk of secondary contamination. The cellulose nitrate (cellulose ester) membrane combines effective retention of microorganisms with high flow rates and optimal colony growth. Its printed grid facilitates colony counting, especially in the case of microcolonies and relatively high numbers of colony-forming units.

Easy Use with the Greatest Possible Flexibility

Microbiological analysis using Microsart® @filter is exceptionally easy: after samples are taken, the sterile filter unit is removed from the package and attached to a filter station. The transparent filter units with graduated marks on their funnels enable users to readily check the filling volume and the progress of filtration.

The Click-Fit closure on the filter unit can be easily opened and the funnel quickly removed. In addition, the membrane can be conveniently removed as the filter base has notches for the forceps. After filtration, the membrane filter is placed on a petri dish with an agar culture medium that is specifically formulated for the microorganisms to detect, and the colony-forming units are later counted. Microsart® @filter products are ideal for sample volumes of up to 100 ml and 250 ml and their membranes are available in a choice of pore sizes and filter colors.

Links

Tags

Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.