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New Probes for Directly Detecting Nucleotide-Protein Interactions
23 Aug 2010Sarah Sarah
Marketing / Sales
Product news
Mant-ATP and Mant-GTP from AnaSpec are the fluorescent analogs of ATP and GTP where either the ribose 2’-hydroxy or the 3’-hydroxy group is esterified by the fluorescent methylisatoic acid. The fluorescence quantum yield of Mant fluorophore is 0.2 in water, which increases significantly in nonpolar solvents or upon binding to most proteins. The highly environmentally sensitive fluorescence of Mant makes Mant-ATP and Mant-GTP useful probes for directly detecting nucleotide-protein interactions at Ex/Em=355/448 nm.
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.