SelectScience
Join Free
Accelerating Science
NewsLife Sciences

New Primary Cell Isolation and Tissue Dissociation Guide Available

Worthington Biochemical Corp.
3 Dec 2012

Product news

Visit Worthington booth #500 at ASCB 2012 to complete a short ‘Primary Cell Isolation’ survey and enter a drawing to win an iPod.

Also request a copy of Worthington's new Tissue Dissociation Guide that is available to assist researchers with selecting enzymes, developing and optimizing protocols and better understanding the process of enzymatic primary cell isolation and tissue dissociation. The guide discusses the enzymes most commonly used, their handling and specificity, and includes hundreds of new detailed cell and species-specific references.

The new Worthington Hepatocyte Isolation System has been developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system. By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes. In addition, Worthington use-tests each lot by isolating hepatocytes from adult rat to assure performance, reliability, and consistent yield of viable cells. As in our Neonatal Cardiomyocyte Isolation and Neural (Papain) Dissociation Kits, each kit includes single-use enzyme vials, balanced salt solution, buffer, culture media and an optimized protocol for five separate adult rat liver perfusions.

FREE Collagenase Sampling Program
As the leading manufacturer of collagenases, Worthington is able to offer the greatest number of different lots at any given time and recommend specific lots for an application. Under the program, individual researchers are provided with 100 mg samples of up to three different lots of collagenase for evaluation in their own assay systems. A period of 60 days is allowed for your evaluation of these samples. A minimum of 3 grams of each lot will be placed on HOLD, reserved in your name. When you determine which lot performs best for you, simply specify the lot desired when ordering. Samples and our Online Lot Selection Tool are available through our website.

Links

Worthington Biochemical Corp.Company website

Tags

Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.CardiomyocytesTissue ProcessingTissue processing involves preparing biological tissue samples for microscopic analysis, including fixation, embedding, and sectioning. This process is essential for histopathology and diagnostic applications. Explore tissue processing products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.HepatocytesCollagenASCBCell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.

Related articles

New Primary Cell Isolation and Tissue Dissociation Guide Available