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New Platform for Faster Separation and Quantification of Metabolites in Rapeseeds

Thermo Fisher Scientific
11 Jan 2011
Sarah Sarah
Marketing / Sales

Product news

Dionex announces a new platform developed to measure various metabolites in Brassica napus seeds. This method combines separation using the 1 mm IonSwift™ MAX-100 anion-exchange column and ICS-3000 system and detection with the API 4000™ electrospray ionization (ESI) tandem mass spectrometry (MS/MS) system from AB SCIEX.

This combination provides a simple, rapid, specific, and sensitive method for the simultaneous detection and quantification of several primary metabolites involved in the tricarboxylic acid (TCA) cycle and glycolysis pathway. Compared to the previously used method, the IonSwift MAX-100 column facilitates shorter run times and helps achieve better sensitivity.

This new platform combines Dionex ion chromatography (IC) and triple quadrupole MS. The IC tandem mass spectrometry (IC-MS/MS) technique provides a good tool to identify and quantify metabolites in a complex matrix. The biological pathways include many homologs and structural isomers with the same mass and analog fragmentations, so it is essential for the analysis to provide good resolution and specific identification of the different compounds.

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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.

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New Platform for Faster Separation and Quantification of Metabolites in Rapeseeds