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New PCR Reagent Kit Accelerates Next-Generation Sequencing

Cambio Ltd
22 May 2011
bridget bridget
Laboratory Director

Product news

Available exclusively in the UK from Cambio, the new HaloPlex™ Target Enrichment Kit revolutionises next-generation sequencing, reducing costs and saving up to 80 per cent in sample preparation time compared to many other kits.

HaloPlex Target Enrichment Kit introduces a dramatically improved PCR technology that enables millions of PCR reactions in a single tube. Following fragmentation using specifically selected restriction endonucleases, and denaturing of the DNA sample, a Selector Probe library is added. Each probe, designed to hybridize to both ends of a targeted DNA restriction fragment, guides the targeted fragments to form circular DNA molecules. Only circular DNA targets are amplified, ready for sequencing using any next-generation procedure, and results are highly reproducible.

According to Dr Naeem Ahmed, Cambio’s technical support specialist, ‘This new Halo Genomics kit provides a “lab-in-a-tube” solution which greatly simplifies PCR workflow, with no need for expensive instrumentation or automation. With next-generation nano scale PCR, reactions are parallelised at the molecular level so that no separate time-consuming library preparation stage is required. For a project targeting 100 genes in 1000 samples, HaloPlex Target Enrichment Kit reduces the total sample preparation time by as much as 80 per cent. Genomic regions of interest are enriched at the same time as the introduction of sample barcoding and sequence motifs.’

Current applications demonstrating the value of the HaloPlex PCR reagent kit for high performance targeted resequencing include studies of candidate genes involved in rare inherited diseases, pathway sequencing in matched tumour and normal samples, and clinical sequencing for BRCA gene assays.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.

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New PCR Reagent Kit Accelerates Next-Generation Sequencing