New On-Demand Webinar from AMSBIO Discusses DNA Damage in Individual Cells

29 Sept 2013
Sarah Thomas
Associate Editor

Product news

AMSBIO has announced a new on-demand webinar entitled 'CometAssay: Detecting single and double strand DNA breaks in individual cells'. Presented by Dr. Sandra Woodgate, a Subject Matter Expert for this technique, this informative webinar explores how the Comet Assay quantitates DNA damage and repair from in vivo and in vitro samples of eukaryotic cells.

The comet assay or single cell gel electrophoresis (SCGE) is a technique to easily and rapidly detect and quantitate DNA fragmentation associated with DNA damage caused by apoptosis and cytotoxicity. The technique can be particularly useful in the field of genotoxicity testing, human biomonitoring, cellular response to DNA damage, and for screening cancer therapeutics.

In this recorded webinar, Dr. Woodgate provides an informative introduction to the Comet Assay, how it works and how standardized CometAssay technology offers a unique system for reproducibly measuring DNA damage. Further the webinar includes real application data, demonstrating the use of CometAssay techniques for studying cellular response to DNA damage, as well as to screen inhibitors to DNA repair, test chemicals for toxicity and screen samples for environmental mutagens.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.ApoptosisApoptosis, or programmed cell death, is a vital process for healthy tissues and is key to our understanding of disease mechanisms like cancer and neurodegeneration. Research tools enable apoptosis detection, quantification, and analysis. Browse products for apoptosis research in our peer-reviewed product directory.GenotoxicityCytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.DNA Damage