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New Nano LC System with ProFlow Technology Offers Advanced Low-Flow Performance

The Thermo Scientific UltiMate 3000 RSLCnano with ProFlow technology provides high-quality analyte identification and quantitation

27 Jan 2016
Alex Waite
Editorial Assistant

Product news

Scientists performing nano liquid chromatography/mass spectrometry (LC-MS) analyses who seek accurate quantitation and analyte identification can now use a system designed to deliver higher retention time precision and ease of use.

The latest version of the Thermo Scientific UltiMate 3000 RSLCnano UHPLC system combines higher nano LC performance with versatility and ease-of-use. New ProFlow technology increases retention time precision, resulting in high-quality data with accurate quantitation. Additionally, the system offers reduced start-up time and simplified system operation so that researchers can focus more time on data acquisition and analysis.

“Life scientists are pushing research boundaries, but they also rely on nano flow technology to enable more productive and efficient workflows, said Remco Swart, Ph.D., director, LC-MS technologies, Thermo Fisher. Our new nano UHPLC system combines the precision of ProFlow technology with ease of operation so that researchers can focus less on the technology and more on their work.

The UltiMate 3000 RSLCnano offers a wide flow range (nano-to-micro flow

rates) in UHPLC mode as well as an additional micro-to-analytical flow ternary gradient pump for increased versatility. As a result, almost any low-flow workflow can benefit from this system. Dedicated kits are available for many standard and advanced (e.g. two-dimensional) workflows.

All connections use Thermo Scientific nanoViper fingertight fittings to ensure rapid and easy set-up and virtually zero-dead-volume connectivity.

Additionally, dedicated software is available to integrate the system with Thermo Scientific mass spectrometers.

“ProFlow technology allows long-term nano LC-MS operation with less maintenance and the highest performance,” said Mark Larance, Ph.D., University of Dundee in Scotland, after testing the new technology.

The UltiMate 3000 RSLCnano HPLC system will be exhibited in booth 10 at the 14th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology in Ghent, Belgium. The event takes place Jan. 27-29 2016.

Dr. Swart will introduce the new features of the system in a seminar entitled “New Nano LC Performance Levels for Proteomics Workflows,” which takes place on Jan. 28 from 1:30-2:15 p.m.

More information on the UltiMate 3000 RSLCnano can be found at www.thermoscientific.com/RSLCnano.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.LC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.

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