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New nano-Advance UHPLC for Proteomics

21 May 2012
Roger Wayman
Administrator / Office Personnel

Product news

At ASMS 2012, Bruker announced the introduction of its new nano-Advance UHPLC system for capillary and nano-flow LC-MS applications. Combining the novel nano-Advance UHPLC with Bruker's leading mass spectrometry platforms, including the amaZon Ion Trap series and maxis UHR-TOF series, further enhances Bruker's integrated solutions for proteomics.

The new nano-Advance UHPLC delivers reproducible retention times with accurate gradients down to 50nL/min. With run-to-run sample carryover typically less than 0.005%, the nano-Advance UHPLC delivers a high level of separation performance critical to the success of many proteomics workflows. The nano-Advance UHPLC was specifically designed to eliminate dead volume and gradient delay wherever possible. When combined with Bruker's CaptiveSpray Ion source, the combination can deliver up to twice the MS utilization rate of typical nano-LC systems coupled to nanospray MS sources. The increased mass spectrometry utilization rate enables more proteins and peptides to be identified and quantified in a single LC-MS run. This is especially significant for detection of low abundance proteins in complex samples, which are easily missed due to the inability of the mass spectrometer to keep up with significant numbers of co-eluting peptides.

The nano-Advance UHPLC delivers precise nanoliter-scale flow rates without the need for any split-flow due to the incorporation of two high performance direct drive syringe pumps. There is also a third pump for trap loading and 2D-LC applications. In conjunction with the CaptiveSpray Ion source, the nano-Advance loading capacity is significantly higher than for conventional nano-LC systems, resulting in higher dynamic range and increased protein coverage and identification.

Dr. Kerry Nugent, Bruker's Senior Vice President - Liquid Chromatography, commented: "With the introduction of the nano-Advance UHPLC, Bruker has taken another step to expand our position as a leading provider of mass spectrometry-based proteomics solutions. The ability of the nano-Advance UHPLC, in conjunction with the CaptiveSpray Ion source, to deliver increased MS productivity, enables the identification of more proteins with greater confidence in the same period of time."

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ASMSThe American Society for Mass Spectrometry (ASMS) is an organization dedicated to advancing the science and application of mass spectrometry. ASMS supports professionals through events, publications, and research initiatives, promoting innovation in areas like proteomics, metabolomics, and environmental analysis.

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New nano-Advance UHPLC for Proteomics