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New Monoclonal Antibody Characterization Platform Analyzes over 1000 Samples Per Day

Thermo Fisher Scientific
16 Mar 2011
bridget bridget
Laboratory Director

Product news

Dionex, the leader in monoclonal antibody analysis solutions, is pleased to announce the release of a new Dionex Monoclonal Antibody (MAb) Characterization Platform, built on the proven Dionex UltiMate® 3000 HPLC system. With an increasing demand for newer and better MAb candidates, there is a bottleneck in the MAb characterization process which is addressed by the Dionex MAb platform.

The Dionex MAb Characterization Platform automates the three manual steps required in the screening and analysis of MAbs, and thus speeds up product development by increasing sample throughput. The fully biocompatible platform features a dual ternary gradient pump design and an integrated fraction collector with the ability to automatically re-inject samples into the second dimension.

Applications that could benefit from these features include automation of purification and analysis of hundreds of MAb samples.

The off-line column regeneration feature in tandem configuration allows the analysis of over 1000 MAb samples a day while the dual column option in parallel configuration allows multiple analyses to be performed on the same sample simultaneously.

New columns for this platform include:
• MabPac™ SCX-10 cation exchange columns for the high resolution and characterization of MAb variants plus new three new column formats: 4 × 150 mm for fast MAb separations; 4 × 50 mm column for pH gradients; and 2 × 150 mm for upstream separations in LC/MS applications.
• MAbPac SEC-1 size exclusion chromatography columns for the separation and analysis of MAbs, their aggregates, and fragments.

For more information, visit the company article page.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.

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New Monoclonal Antibody Characterization Platform Analyzes over 1000 Samples Per Day