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New Magnetic Beads for Affinity Binding Techniques from Thermo Fisher Scientific

18 Aug 2011
Roger Wayman
Administrator / Office Personnel

Product news

Thermo Fisher Scientific have announced the release of Thermo Scientific Pierce Protein A/G Magnetic Beads and Pierce Protein A/G Magnetic IP/Co-IP Kits. These new products provide a fast, convenient method for manual and automated magnetic isolation and immunoprecipitation (IP) of proteins using affinity binding. The beads are validated for numerous techniques including IP, co-immunoprecipitation (co-IP), gel electrophoresis, Western blotting and mass spectrometry.

The Pierce® Protein A/G Magnetic Beads are iron oxide particles covalently coated with a monolayer of Protein A/G. The beads offer a high binding capacity of 55-85 micrograms of rabbit IgG per milligram of magnetic particles, with low non-specific background. Pierce Protein A/G Magnetic Beads are easily removed from the solution manually using a magnetic stand or by automation using equipment such as the Thermo Scientific KingFisher Flex Instrument.

The Pierce Protein A/G Magnetic Beads are available alone or as part of a comprehensive kit for IP or co-IP methods.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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