New Low-Cost PCR-Amplified cDNA Sequencer Revealed

28 Feb 2019
Matthew Horan
Consultant

Product news

Dolomite Bio, a leading provider of cutting-edge single cell technologies, has announced the launch of a new scRNA-Seq Reagent Kit to expand the Nadia Instrument portfolio. This convenient and cost-effective option is being showcased at the Advances in Genome Biology and Technology 2019 General Meeting (AGBT2019) – February 27th to March 2nd in Marco Island, Florida. The launch of this new reagent kit demonstrates Dolomite Bio’s commitment to the continuous development of the Nadia product family, and provides customers with a high quality, straightforward single cell profiling solution while still offering them the flexibility to use their own reagents if desired.

The new scRNA-Seq Reagent Kit is designed to enable robust and reliable generation of PCR-amplified cDNA libraries on the Nadia Instrument, allowing high throughput gene expression profiling of heterogenous cell populations. The kit contains all the consumables required to create amplified cDNA libraries, allowing researchers to achieve highly reproducible single cell data sets – with ultra-efficient gene capture rates – at low sequencing costs. Unlike other solutions, this set¬up also gives users the ability to adjust the number of single cell cDNA libraries prior to sequencing, helping to ensure cost-efficient single cell analysis.

scRNA-Seq Reagent Kit

Combined with the Nadia Instrument, the scRNA-Seq Reagent Kit generates PCR amplified cDNA libraries for high throughput gene expression profiling of heterogenous cell populations in just 20 minutes. The kit allows researchers to achieve highly reproducible single cell data sets – with ultra-efficient gene capture rates – at low sequencing costs. The kit contains all the reagents and consumables required for the generation of 8 PCR amplified cDNA libraries. These include: Cell and bead suspension buffers Barcoded beads Emulsion oil Perfluorooctanol (PFO) Bead wash buffers Reverse transcription reaction mixE xonuclease reaction mix PCR reaction mix

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