New Line of Kits Simplify Protein Extraction from Cells and Tissues

3 Dec 2010
Sarah Sarah
Marketing / Sales

Product news

Fast and easy to use, individual DUALXtract kits from Dualsystems Biotech AG are available for total protein extraction, the extraction of integral membrane proteins and membrane-associated proteins and the isolation of cytoplasmic and nuclear proteins.

DUALXtract Mammalian Cell Total Protein Extraction Reagent provides gentle one-step extraction of total protein, while the DUALXtract Nuclear and Cytoplasmic Protein Extraction Kit rapidly isolates cytoplasmic and nuclear proteins from cultured cells and tissues. Both are ideal for use with downstream applications such as 1D and 2D gel electrophoresis, Western blotting, electrophoretic mobility shift assay (EMSA), immunoprecipitation, affinity purification, and enzymatic activity and reporter gene assays. The DUALXtract Total Membrane Protein Extraction Kit quickly isolates high quality membrane and cytoplasmic protein fractions from mammalian cells and tissues, with no cross-contamination. The DUALXtract Membrane Protein Optimization Kit can be used to quickly identify optimal extraction conditions for membrane proteins of interest and is suitable for the extraction of native, functional membrane proteins from mammalian, fungal, and bacterial cells.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.