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New Kits Enable High Yield Purification of gDNA from Challenging Blood and Tissue Samples

Promega Corp.
18 Jan 2011
Sarah Sarah
Marketing / Sales

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The ReliaPrep™ Blood and Tissue gDNA Miniprep Systems from Promega Corporation enable the extraction of high yields of very pure gDNA from even the most challenging samples, such as frozen blood. The new kits have been designed for use with small sample volumes and purify clean gDNA without ethanol, which can inhibit downstream applications.

The ReliaPrep Blood gDNA Miniprep System purifies intact gDNA from up to 200µl of blood or body fluid. Genomic DNA can be prepared from fresh or frozen blood in less than 40 minutes, with expected DNA yields of 4–10µg, depending on the white blood cell count of the blood sample. The ReliaPrep gDNA Tissue Miniprep System purifies gDNA from up to 25mg of tissue. Both systems are complete and ready to use, producing consistent results from run to run and between users even with haemolysed samples, without the use of alcohol washes or precipitations.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.HematologyIn Haematology / Hematology, complete blood cell counts (or full blood counts) are obtained using automated blood count analyzers to enumerate blood cell types.  Hematology also encompasses haemostasis and coagulation, thrombophilia and hemophilia, plasma viscosity and ESR analysis, hemoglobinopathies, cell morphology and haematinic measurement.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.

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New Kits Enable High Yield Purification of gDNA from Challenging Blood and Tissue Samples